Altered Topographic Distribution and Enhanced Neuronal Expression of Adenosine-Metabolizing Enzymes in Rat Hippocampus and Cortex from Early to late Adulthood
| العنوان: | Altered Topographic Distribution and Enhanced Neuronal Expression of Adenosine-Metabolizing Enzymes in Rat Hippocampus and Cortex from Early to late Adulthood |
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| المؤلفون: | Milorad Dragic, Andjela Stekic, Milica Zeljkovic, Marina Zaric Kontic, Katarina Mihajlovic, Marija Adzic, Ivana Grkovic, Nadezda Nedeljkovic |
| المصدر: | Neurochemical Research |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Male, Neurons, Adenosine, Receptor, Adenosine A2A, Adenosine deaminase (ADA), General Medicine, Hippocampus, Biochemistry, Rats, Cellular and Molecular Neuroscience, ecto-5ʹ-nucleotidase/CD73, Tissue non-specific alkaline phosphatase (TNAP), Synapses, Animals, A2AR, 5'-Nucleotidase |
| الوصف: | The present study demonstrates altered topographic distribution and enhanced neuronal expression of major adenosine-metabolizing enzymes, i.e. ecto-5ʹ-nucleotidase (eN) and tissue non-specific alkaline phosphatase (TNAP), as well as adenosine receptor subtype A2A in the hippocampus and cortex of male rats from early to late adulthood (3, 6, 12 and 15 months old males). The significant effect of age was demonstrated for the increase in the activity and the protein expression of eN and TNAP. At 15-m, enzyme histochemistry demonstrated enhanced expression of eN in synapse-rich hippocampal and cortical layers, whereas the upsurge of TNAP was observed in the hippocampal and cortical neuropil, rather than in cells and layers where two enzymes mostly reside in 3-m old brain. Furthermore, a dichotomy in A1R and A2AR expression was demonstrated in the cortex and hippocampus from early to late adulthood. Specifically, a decrease in A1R and enhancement of A2AR expression were demonstrated by immunohistochemistry, the latter being almost exclusively localized in hippocampal pyramidal and cortical superficial cell layers. We did not observe any glial upregulation of A2AR, which was common for both advanced age and chronic neurodegeneration. Taken together, the results imply that the adaptative changes in adenosine signaling occurring in neuronal elements early in life may be responsible for the later prominent glial enhancement in A2AR-mediated adenosine signaling, and neuroinflammation and neurodegeneration, which are the hallmarks of both advanced age and age-associated neurodegenerative diseases. |
| تدمد: | 1573-6903 0364-3190 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3573b2433c68d7dd18c2c729de73bffbTest https://doi.org/10.1007/s11064-022-03557-5Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....3573b2433c68d7dd18c2c729de73bffb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15736903 03643190 |
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