Limiting the pathogenesis of Salmonella Typhimurium with berry phenolic extracts and linoleic acid overproducing Lactobacillus casei
| العنوان: | Limiting the pathogenesis of Salmonella Typhimurium with berry phenolic extracts and linoleic acid overproducing Lactobacillus casei |
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| المؤلفون: | Debabrata Biswas, Puja Patel, Zajeba Tabashsum, Cassendra Bernhardt, Mengfei Peng, Michael Carrion, Shaik O. Rahaman |
| المصدر: | Journal of Microbiology. 58:489-498 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Salmonella typhimurium, Lactobacillus casei, medicine.medical_treatment, Linoleic acid, Conjugated linoleic acid, Virulence, Applied Microbiology and Biotechnology, Microbiology, Cell Line, Linoleic Acid, 03 medical and health sciences, chemistry.chemical_compound, medicine, Animals, Humans, Pathogen, 030304 developmental biology, 0303 health sciences, biology, Plant Extracts, 030306 microbiology, Probiotics, Prebiotic, food and beverages, General Medicine, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, Lacticaseibacillus casei, Prebiotics, chemistry, Salmonella enterica, Fruit |
| الوصف: | The growing threat of emergent multidrug-resistant enteric bacterial pathogens, and their adopted virulence properties are directing to find alternative antimicrobials and/or development of dietaries that can improve host gut health and/or defense. Recently, we found that modified Lactobacillus casei (Lc + CLA) with increased production of conjugated linoleic acid has antimicrobial and other beneficial properties. Further, prebiotic alike products such as berry pomace extracts (BPEs), increase the growth of probiotics and inhibit the growth of certain bacterial pathogens. In this study, we evaluated the antibacterial effect of genetically modified Lc + CLA along with BPEs against major enteric pathogen Salmonella enterica serovar Typhimurium (ST). In mixed culture condition, the growth of ST was significantly reduced in the presence of Lc + CLA and/or BPEs. Bacterial cell-free cultural supernatant (CFCS) collected from wild-type Lc or modified Lc + CLA strains also inhibited the growth and survival of ST, and those inhibitory effects were enhanced in the presence of BPEs. We also found that the interaction of the pathogen with cultured host (HD-11 and INT-407) cells were also altered in the presence of either Lc or Lc + CLA strain or their CFCSs significantly. Furthermore, the relative expression of genes related to ST virulence and physicochemical properties of ST was altered by the effect of CFCSs of either Lc or Lc + CLA. These findings indicate that a diet containing synbiotic, specifically linoleic acid, over-produced Lc + CLA and prebiotic product BPEs, might have the potential to be effective in controlling ST growth and pathogenesis. |
| تدمد: | 1976-3794 1225-8873 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68966687c3ce4c21e2d16dced96eb72cTest https://doi.org/10.1007/s12275-020-9545-1Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....68966687c3ce4c21e2d16dced96eb72c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19763794 12258873 |
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