Measurement uncertainty of 25-OH vitamin D determination with different commercially available kits: impact on the clinical cut offs
| العنوان: | Measurement uncertainty of 25-OH vitamin D determination with different commercially available kits: impact on the clinical cut offs |
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| المؤلفون: | Romy Gadisseur, Jean-Claude Souberbielle, M. Monge, Eric Rozet, Pierre Delanaye, Philippe Hubert, Agnès Carlisi, Jean Paul Chapelle, Etienne Cavalier |
| المصدر: | Osteoporosis International. 21:1047-1051 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | medicine.medical_specialty, Chromatography, business.industry, Endocrinology, Diabetes and Metabolism, Vitamin D Deficiency, Target concentration, Normal limit, chemistry.chemical_compound, Endocrinology, Blood serum, chemistry, Reference Values, Reference values, Internal medicine, Vitamin D and neurology, Humans, Medicine, Measurement uncertainty, Calcifediol, Reagent Kits, Diagnostic, Vitamin D, business, Chromatography, High Pressure Liquid |
| الوصف: | Due to “measurement uncertainty”, the “true” 25-OH vitamin D (25(OH)D) of a patient (whatever the commercially available assay tested) will be >80 nmol/L if its measured concentration is >100 nmol/L. Thus, if a physician considers that a normal VTD status is a 25(OH)D level ≥80 nmol/L, he should ensure that the patient’s results are ≥100 nmol/L. Many experts recommend that serum levels of 25(OH)D should be above a lower normal limit of 75–80 nmol/L. However, the value delivered by laboratories is only an estimation of the “true” value due to “measurement uncertainty.” When using a cut off, measurement uncertainty around the cut off is important because therapeutic actions may differ if the measured value is below or above the limit. We aimed to establish the “measurement uncertainty” at different levels of concentration for several commercially available 25(OH)D analytical techniques. We constituted three pools of serum with different 25(OH)D concentrations. Each pool was assayed in triplicate during 5 days with the DiaSorin RIA, Liaison, Elecsys, and Chromsystems-HPLC assays. We report a relatively high “measurement uncertainty” for the measurement of 25(OH)D for the four different techniques: the mean relative uncertainties, all techniques confounded were 19.4%, 16.0%, and 11.3% for pool 1 (35.3 nmol/L), pool 2 (79.5 nmol/L), and pool 3 (126.1 nmol/L), respectively. Our results show that, whatever the assay, the “true” 25(OH)D of a patient will be >80 nmol/L if its measured concentration is >100 nmol/L. In other words, if a physician considers that a normal VTD status is defined by a 25(OH)D level ≥80 nmol/L, he should ensure that the patients present a 25(OH)D ≥100 nmol/L. |
| تدمد: | 1433-2965 0937-941X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6487f293528295ff4e37bd80fa001894Test https://doi.org/10.1007/s00198-009-1052-5Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6487f293528295ff4e37bd80fa001894 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14332965 0937941X |
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