دورية أكاديمية
Differential location of NKT and MAIT cells within lymphoid tissue
| العنوان: | Differential location of NKT and MAIT cells within lymphoid tissue |
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| المؤلفون: | Johnson, Darryl N., Ruan, Zheng, Petley, Emma V., Devi, Sapna, Holz, Lauren E., Uldrich, Adam P., Mak, Jeffrey Y. W., Hor, Jyh Liang, Mueller, Scott N., McCluskey, James, Fairlie, David P., Darcy, Phillip K., Beavis, Paul A., Heath, William R., Godfrey, Dale I. |
| المساهمون: | Australian Research Council Center of Excellence in Advanced Molecular Imaging, National Health and Medical Research Council |
| المصدر: | Scientific Reports ; volume 12, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | Natural Killer T (NKT) cells and Mucosal-Associated Invariant T (MAIT) cells are innate-like T cells that express semi-invariant αβ T cell receptors (TCRs) through which they recognise CD1d and MR1 molecules, respectively, in complex with specific ligands. These cells play important roles in health and disease in many organs, but their precise intra-organ location is not well established. Here, using CD1d and MR1 tetramer staining techniques, we describe the precise location of NKT and MAIT cells in lymphoid and peripheral organs. Within the thymus, NKT cells were concentrated in the medullary side of the corticomedullary junction. In spleen and lymph nodes, NKT cells were mainly localised within T cell zones, although following in vivo activation with the potent NKT-cell ligand α-GalCer, they expanded throughout the spleen. MAIT cells were clearly detectable in Vα19 TCR transgenic mice and were rare but detectable in lymphoid tissue of non-transgenic mice. In contrast to NKT cells, MAIT cells were more closely associated with the B cell zone and red pulp of the spleen. Accordingly, we have provided an extensive analysis of the in situ localisation of NKT and MAIT cells and suggest differences between the intra-organ location of these two cell types. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-022-07704-4 |
| الإتاحة: | https://doi.org/10.1038/s41598-022-07704-4Test https://www.nature.com/articles/s41598-022-07704-4.pdfTest https://www.nature.com/articles/s41598-022-07704-4Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.FEA94CD4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-022-07704-4 |
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