Small extracellular vesicles derived from human MSCs prevent allergic airway inflammation via immunomodulation on pulmonary macrophages
| العنوان: | Small extracellular vesicles derived from human MSCs prevent allergic airway inflammation via immunomodulation on pulmonary macrophages |
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| المؤلفون: | Zi-Cong Wu, Bi-Xin He, Ya-Qi Peng, Qing-Ling Fu, Zhi-Bin Xu, Xing-Liang Fan, Shu-Bin Fang, Song Guo Zheng, Cong Wang, Zhang-Jin Wu, Xiang-Ci Meng, Hong-Yu Zhang |
| المصدر: | Cell Death & Disease Cell Death and Disease, Vol 11, Iss 6, Pp 1-15 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Proteome, viruses, Induced Pluripotent Stem Cells, Immunology, Spleen, Proteomics, Models, Biological, Article, Immunomodulation, Extracellular Vesicles, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, In vivo, Hypersensitivity, medicine, Animals, Humans, lcsh:QH573-671, Lung, Inflammation, Mice, Inbred BALB C, medicine.diagnostic_test, lcsh:Cytology, business.industry, Macrophages, Mesenchymal stem cell, Cell Polarity, virus diseases, Cell Differentiation, Mesenchymal Stem Cells, Cell Biology, respiratory system, medicine.disease, Asthma, In vitro, 030104 developmental biology, Bronchoalveolar lavage, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Female, business, Infiltration (medical) |
| الوصف: | Allergic airway inflammation is a major public health disease that affects up to 300 million people in the world. However, its management remains largely unsatisfactory. The dysfunction of pulmonary macrophages contributes greatly to the development of allergic airway inflammation. It has been reported that small extracellular vesicles derived from mesenchymal stromal cells (MSC-sEV) were able to display extensive therapeutic effects in some immune diseases. This study aimed to investigate the effects of MSC-sEV on allergic airway inflammation, and the role of macrophages involved in it. We successfully isolated MSC-sEV by using anion exchange chromatography, which were morphologically intact and positive for the specific EV markers. MSC-sEV significantly reduced infiltration of inflammatory cells and number of epithelial goblet cells in lung tissues of mice with allergic airway inflammation. Levels of inflammatory cells and cytokines in bronchoalveolar lavage fluid were also significantly decreased. Importantly, levels of monocytes-derived alveolar macrophages and M2 macrophages were significantly reduced by MSC-sEV. MSC-sEV were excreted through spleen and liver at 24 h post-administration in mice, and were able to be taken in by macrophages both in vivo and in vitro. In addition, proteomics analysis of MSC-sEV revealed that the indicated three types of MSC-sEV contained different quantities of proteins and shared 312 common proteins, which may be involved in the therapeutic effects of MSC-sEV. In total, our study demonstrated that MSC-sEV isolated by anion exchange chromatography were able to ameliorate Th2-dominant allergic airway inflammation through immunoregulation on pulmonary macrophages, suggesting that MSC-sEV were promising alternative therapy for allergic airway inflammation in the future. |
| تدمد: | 2041-4889 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db8f8fa80d27d4d886c97fbfbbc6f897Test https://doi.org/10.1038/s41419-020-2606-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....db8f8fa80d27d4d886c97fbfbbc6f897 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20414889 |
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