Retinoic acid receptor β2 re-expression and growth inhibition in thyroid carcinoma cell lines after 5-aza-2′-deoxycytidine treatment
| العنوان: | Retinoic acid receptor β2 re-expression and growth inhibition in thyroid carcinoma cell lines after 5-aza-2′-deoxycytidine treatment |
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| المؤلفون: | Aldo Pinchera, Alessandra Capodanno, Paola Collecchi, Laura Agate, Fy Miasaki, Agnese Vivaldi, Raffaele Ciampi, Rossella Elisei |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | Springer Science and Business Media LLC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Antimetabolites, Antineoplastic, Receptors, Retinoic Acid, Endocrinology, Diabetes and Metabolism, Cell, Thyroid Gland, Retinoic acid, Biology, Decitabine, Transfection, chemistry.chemical_compound, Endocrinology, Cell Line, Tumor, medicine, Humans, Thyroid Neoplasms, Promoter Regions, Genetic, Cell Proliferation, Cell growth, Retinoic Acid Receptor alpha, Carcinoma, DNA Methylation, Molecular biology, Demethylating agent, Gene Expression Regulation, Neoplastic, Retinoic acid receptor, medicine.anatomical_structure, chemistry, Organ Specificity, Cell culture, Apoptosis, Azacitidine, Cancer research, Growth inhibition |
| الوصف: | The treatment of both undifferentiated and de-differentiated thyroid tumors, which are unresponsive to radioiodine, represents one of the biggest challenges for thyroidologists. The aim of the present study was to investigate in vitro the methylation status of retinoic acid receptors (RAR)beta2 promoter and the effect of the demethylating agent 5-aza-2'-deoxycytidine (5-Aza-CdR) on 5 human thyroid cancer cell lines. The methylation status of RARbeta2 promoter was analyzed by methylation-specific PCR. The effect of 5-Aza-CdR on cell growth and apoptosis was evaluated by cell counting, enzymelinked immunosorbent assay tests and fluorescence-activated cell sorting analysis, while the effect on the expression of RAR and thyroid-specific genes was measured by qualitative and quantitative RT-PCR. Methylation of RARbeta2 promoter was present only in ARO cells. 5-Aza-CdR determined growth inhibition in all cell lines, probably due to apoptosis in WRO, NPA, and ARO cells, and to inhibition of DNA synthesis in TT cells. Treatment with 5-Aza-CdR induced the expression of RARbeta mRNA in ARO and FRO cells, a slight increase of the expression of Tg, TPO and thyroid trancription factor 1 (TTF-1) mRNA and the new expression of low levels of NIS in TT cells. A significant increase of TTF-1 mRNA in FRO cells was also observed. In this study we demonstrated that RARbeta2 promoter was methylated in ARO cell line. However, the 5-Aza-CdR treatment induced RARbetamRNA expression not only in ARO but also in FRO and TT cell lines, whose RARbeta2 promoter was unmethylated. A significant reduction of cell growth, but not cell re-differentiation, was also observed after 5-Aza-CdR treatment. |
| تدمد: | 1720-8386 0391-4097 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::057b5a0b197cb3ec12e8f0d9f9284deeTest https://doi.org/10.1007/bf03346422Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....057b5a0b197cb3ec12e8f0d9f9284dee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17208386 03914097 |
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