Isoflavone synthase (IFS) gene phylogeny in Trifolium species associated with plant isoflavone contents
| العنوان: | Isoflavone synthase (IFS) gene phylogeny in Trifolium species associated with plant isoflavone contents |
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| المؤلفون: | J. Harris, Manuel Curto, M. Graça Pereira, Eugénia Nunes, Tatiana Visnevschi-Necrasov, Miguel A. Faria, Sara C. Cunha, Harald Meimberg, M. Beatriz P.P. Oliveira |
| المصدر: | Plant Systematics and Evolution. 299:357-367 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Daidzein, Pratensein, Plant Science, Glycitein, Biology, Biochanin A, chemistry.chemical_compound, Irilone, chemistry, Botany, Genistin, Formononetin, Daidzin, Ecology, Evolution, Behavior and Systematics |
| الوصف: | This study presents the results of the identification and quantification of 12 isoflavones (prunetin, irilone, pseudobaptigenin, glycitein, daidzin, genistin, daidzein, pratensein, puerarin, biochanin A, formononetin and genistein) in 23 species of Trifolium (T. arvense, T. pratense, T. ligusticum, T. striatum, T. lappaceum, T. angustifolium, T. hirtum, T. subterraneum, T. isthmocarpum, T. stellatum, T. mutabile, T. strictum, T. fragiferum, T. alexandrinum, T. tomentosum. T. nigrescens subsp. petrisavii, T. nigrescens, T. glomeratum, T. subterraneum subsp. brachycalycinum, T. cherleri, T. resupinatum, T. campestre and T. repens). Isoflavones were extracted by an MSPD method and analyzed with HPLC coupled with a diode-array detector. The evaluation of molecular phylogeny of the IFS gene and the relation with isoflavone content was also performed. Five species (T. subterraneum subsp. brachycalycinum, T. alexandrinum, T. pratense, T. subterraneum and T. lappaceum) were identified with high levels of biochanin A (431–83 mg/kg), formononetin (72–365 mg/kg) and genistein (9–509 mg/kg), which could be utilized as alternative sources for the nutraceutical industry. Genetic phylogeny for the IFS gene was found in the species studied, with 20 out of 23 species having been divided into two clades, while the remaining three were genetically distant. Based on our results, we confirm the direct correlation between IFS gene polymorphism and isoflavones content in species of Trifolium particularly noted for formononetin. Therefore, the IFS gene can be utilized for screening Trifolium genotypes for formononetin. The relation of the three isoflavones' contents and the molecular phylogeny of plants determined by the IFS sequences, as a screening marker for plants with high isoflavone contents in Trifolium species, are to the best of our knowledge described for the first time. |
| تدمد: | 1615-6110 0378-2697 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::4c541da6fff22cee9e4a063526d2ddccTest https://doi.org/10.1007/s00606-012-0726-1Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........4c541da6fff22cee9e4a063526d2ddcc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16156110 03782697 |
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