Acid-sensing ion channel 2 (ASIC2) is selectively localized in the cilia of the non-sensory olfactory epithelium of adult zebrafish
| العنوان: | Acid-sensing ion channel 2 (ASIC2) is selectively localized in the cilia of the non-sensory olfactory epithelium of adult zebrafish |
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| المؤلفون: | José A. Vega, Francesco Abbate, R. Cabo, E. Viña, V. Parisi, J. C. Pérez-Varela, Teresa Cobo, Olivia García-Suárez, Maria Cristina Guerrera, R. Laurà, Luis M. Quirós, Antonino Germanà |
| المصدر: | Histochemistry and Cell Biology. 143:59-68 |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Nasal cavity, Pathology, medicine.medical_specialty, Histology, Sensory system, Olfaction, Biology, Epithelium, Olfactory Mucosa, medicine, Animals, Basal body, Cilia, Molecular Biology, Zebrafish, Acid-sensing ion channel 2, olfactory epithelium, cilium, zebrafish, Cilium, Cell Biology, Hydrogen-Ion Concentration, biology.organism_classification, Cell biology, Acid Sensing Ion Channels, Medical Laboratory Technology, medicine.anatomical_structure, Olfactory epithelium |
| الوصف: | Ionic channels play key roles in the sensory cells, such as transducing specific stimuli into electrical signals. The acid-sensing ion channel (ASIC) family is voltage-insensitive, amiloride-sensitive, proton-gated cation channels involved in several sensory functions. ASIC2, in particular, has a dual function as mechano- and chemo-sensor. In this study, we explored the possible role of zebrafish ASIC2 in olfaction. RT-PCR, Western blot, chromogenic in situ hybridization and immunohistochemistry, as well as ultrastructural analysis, were performed on the olfactory rosette of adult zebrafish. ASIC2 mRNA and protein were detected in homogenates of olfactory rosettes. Specific ASIC2 hybridization was observed in the luminal pole of the non-sensory epithelium, especially in the cilia basal bodies, and immunoreactivity for ASIC2 was restricted to the cilia of the non-sensory cells where it was co-localized with the cilia marker tubulin. ASIC2 expression was always absent in the olfactory cells. These findings demonstrate for the first time the expression of ASIC2 in the olfactory epithelium of adult zebrafish and suggest that it is not involved in olfaction. Since the cilium sense and transduce mechanical and chemical stimuli, ASIC2 expression in this location might be related to detection of aquatic environment pH variations or to detection of water movement through the nasal cavity. |
| تدمد: | 1432-119X 0948-6143 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a557fbe03cbb32e570e3a2d19477d36aTest https://doi.org/10.1007/s00418-014-1264-4Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....a557fbe03cbb32e570e3a2d19477d36a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432119X 09486143 |
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