دورية أكاديمية
miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells
| العنوان: | miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells |
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| المؤلفون: | Sakai, Eiko, Imaizumi, Tsutomu, Suzuki, Ruruka, Taracena-Gándara, Marcos, Fujimoto, Toshiki, Sakurai, Fuminori, Mizuguchi, Hiroyuki |
| المساهمون: | MEXT | Japan Society for the Promotion of Science, Bristol-Myers Squibb Foundation |
| المصدر: | Communications Biology ; volume 6, issue 1 ; ISSN 2399-3642 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology, Medicine (miscellaneous) |
| الوصف: | Non-alcoholic liver disease (NAFLD) is a condition caused by excessive fat accumulation in the liver and developed via multiple pathways. miR-27b has been suggested to play crucial roles in the development of NAFLD, assuming via targeting genes involved in lipid catabolism and anabolism. However, other pathways regulated by miR-27b are largely unknown. Here we show that lipid accumulation was induced in miR-27b –transfected human and mouse hepatic cells and that knockdowns of three miR-27b–target genes, β-1,4-galactosyltransferase 3 ( B4GALT3 ), matrix AAA peptidase interacting protein 1 ( MAIP1 ) and PH domain and leucine rich repeat protein phosphatase 2 ( PHLPP2 ), induced lipid accumulation. We also show that B4GALT3 and MAIP1 were direct targets of miR-27b and overexpression of MAIP1 ameliorated miR-27b −induced lipid accumulation. In addition, we show that hepatic Maip1 expression declined in mice fed a high-fat diet, suggesting the involvement of decreased Maip1 expression in the condition of fatty liver. Overall, we identified MAIP1/miR-27b axis as a mediator of hepatic lipid accumulation, a potential therapeutic target for NAFLD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s42003-023-05049-w |
| الإتاحة: | https://doi.org/10.1038/s42003-023-05049-wTest https://www.nature.com/articles/s42003-023-05049-w.pdfTest https://www.nature.com/articles/s42003-023-05049-wTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.2D857B38 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s42003-023-05049-w |
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