Electrochemical simultaneous assay of chloramphenicol and PCB72 using magnetic and aptamer-modified quantum dot-encoded dendritic nanotracers for signal amplification
| العنوان: | Electrochemical simultaneous assay of chloramphenicol and PCB72 using magnetic and aptamer-modified quantum dot-encoded dendritic nanotracers for signal amplification |
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| المؤلفون: | Meng Chen, Ning Gan, Qing Xu, Huairong Zhang, Qianli Jiang, Zhongdan Yan, Yinji Chen, Tianhua Li |
| المصدر: | Microchimica Acta. 183:1099-1106 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Detection limit, Analyte, Chromatography, Chemistry, Aptamer, 010401 analytical chemistry, Analytical chemistry, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Orders of magnitude (mass), 0104 chemical sciences, Analytical Chemistry, Colloidal gold, Magnetic nanoparticles, Multiplex, 0210 nano-technology, Voltammetry |
| الوصف: | The article describes a multiplex aptamer based voltammetric assay for the food pollutants chloramphenicol (CAP) and polychlorinated biphenyl-72 (PCB72). The method is based on the displacement of a nanotracer (an aptamer-labeled magnetic dendritic probe) by the analytes which cause the release of the corresponding encoded nanotracers. Thiolated aptamers of CAP and PCB72 were first self-assembled on magnetic gold nanoparticles (Fe3O4@Au) as capture probes to concentrate the target analytes CAP and PCB72, respectively. Next, their hybridized complementary strands (cDNA1 or cDNA2) and a dendritic polymerase were linked to quantum dots (CdS or PbS) to form the respective nanotracers. Finally, encoded composite magnetic probes were obtained through hybridization reaction between aptamers and cDNAs. Once CAP and PCB72 are recognized by the composite probes, the nanotracers are released into the supernatant where they can be detected simultaneously by voltammetry of the metal ion markers Cd(II) and Pb(II) at −0.81 and −0.57 V (vs. Ag/AgCl), respectively. The concentration measured for Cd(II) and Pb(II) are directly proportional to the concentrations of CAP and PCB72, respectively, in the range from 0.001 to 100 ng mL−1. The detection limits for CAP and PCB72 are 0.33 and 0.35 pg mL−1, respectively (at an S/N ratio of 3), which is 2 to 3 orders of magnitude lower than the routinely performed and commercially available ELISA. The method is perceived to possess large potential in that it may be applied to other antibiotics by using the appropriate aptamers. |
| تدمد: | 1436-5073 0026-3672 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::da3eae420b42c966ed0970dbe2815e03Test https://doi.org/10.1007/s00604-015-1695-1Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........da3eae420b42c966ed0970dbe2815e03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14365073 00263672 |
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