Antitumor effect of mSurvivinThr34 → Ala in murine colon carcinoma when administered intravenously
| العنوان: | Antitumor effect of mSurvivinThr34 → Ala in murine colon carcinoma when administered intravenously |
|---|---|
| المؤلفون: | Jiong Li, Jian-Rong Xu, Tao Yi, Yongsheng Wang, Ju-mei Zhao, Lian Wang, Hong-Xia Li, Bin Kan, Xiao-xia Du, Yuquan Wei, Yan-jun Wen, Xiao-bo Du, Xing-chen Peng, Xinyu Zhao, Xiang Chen, Bin Ye, Xiancheng Chen, Xia Zhao, Fei Yan |
| المصدر: | Medical Oncology. 27:1156-1163 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Threonine, Cancer Research, Adoptive cell transfer, Colorectal cancer, Angiogenesis, Survivin, Blotting, Western, Apoptosis, CD8-Positive T-Lymphocytes, Inhibitor of Apoptosis Proteins, Immunoenzyme Techniques, Mice, Immune system, Cell Movement, Cell Adhesion, medicine, Animals, Humans, Cationic liposome, Cytotoxicity, Cell Proliferation, Mice, Inbred BALB C, Alanine, Neovascularization, Pathologic, biology, business.industry, Genetic Therapy, Hematology, General Medicine, medicine.disease, Adoptive Transfer, Molecular biology, Repressor Proteins, Oncology, Colonic Neoplasms, Injections, Intravenous, Liposomes, biology.protein, Cancer research, Female, Antibody, business |
| الوصف: | Colorectal cancer is one of the most common cancers. Survivin is strongly immunogenic in a fraction of colorectal cancer patients. The present study was designed to determine whether full-length mouse Survivin dominant-negative mutant SurvivinT34A has the antitumor activity in a murine colon carcinoma model. The complex of cationic liposome (DOTAP/Chol) to plasmid pORF9-mSurvivin T34A was administered intravenously in a mouse subcutaneous (S. C.) CT 26 tumor model. Apoptotic cells and anti-angiogenesis were evaluated by fluorescent in situ TUNEL assay and by immunohistochemistry with an antibody reactive to CD31, respectively. A 4 h 51Cr release assay was performed to determine Survivin-specific cytotoxicity. The adoptive transfer of CD8+ or CD4+ T-lymphocytes assay was to further explore the roles of immune cell subsets. We demonstrated the complex of cationic liposome (DOTAP/Chol) to plasmid pORF9--mSurvivin T34A when administered intravenously induced an efficient antitumor activity in a S. C. CT26 tumor model in mice. The main mechanism is involved in three aspects: triggering the apoptosis of tumor cells, inhibiting angiogenesis, and inducing Survivin-specific immune response. Our observations may have potential implications for the further exploration of the treatment of human colorectal cancer by intravenous delivery of dominant-negative mutant Survivin T34A. |
| تدمد: | 1559-131X 1357-0560 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f72be1b64d534b2138a33832d7aef249Test https://doi.org/10.1007/s12032-009-9353-2Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f72be1b64d534b2138a33832d7aef249 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1559131X 13570560 |
|---|