In vivo cardiomyocyte response to YTX- and AZA-1-induced damage: autophagy versus apoptosis
| العنوان: | In vivo cardiomyocyte response to YTX- and AZA-1-induced damage: autophagy versus apoptosis |
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| المؤلفون: | Cristina Carrera, Sara F. Ferreiro, Luis M. Botana, J. Manuel Cifuentes, Antonio González Cantalapiedra, Andrés Crespo, M. Carmen Louzao, Natalia Vilariño, Germán Santamarina |
| المصدر: | Archives of Toxicology. 91:1859-1870 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Programmed cell death, Time Factors, Health, Toxicology and Mutagenesis, Blotting, Western, Mollusk Venoms, Apoptosis, Biology, Toxicology, Fas ligand, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, In vivo, Autophagy, Animals, Azaspiracid, Myocytes, Cardiac, Spiro Compounds, Oxocins, General Medicine, Rats, Cell biology, Toxicity Tests, Subacute, 030104 developmental biology, chemistry, Female, Marine Toxins, Yessotoxin, Marine toxin, Biomarkers |
| الوصف: | Yessotoxins (YTX) and azaspiracids (AZAs) are marine toxins produced by phytoplanktonic dinoflagellates that get accumulated in filter feeding shellfish and finally reach human consumers through the food web. Both toxin classes are worldwide distributed, and food safety authorities have regulated their content in shellfish in many countries. Recently, YTXs and AZAs have been described as compounds with subacute cardiotoxic potential in rats owed to alterations of the cardiovascular function and ultrastructural heart damage. These molecules are also well known in vitro inducers of cell death. The aim of this study was to explore the presence of cardiomyocyte death after repeated subacute exposure of rats to AZA-1 and YTX for 15 days. Because autophagy and apoptosis are often found in dying cardiomyocytes, several autophagic and apoptotic markers were determined by western blot in heart tissues of these rats. The results showed that hearts from YTX-treated rats presented increased levels of the autophagic markers microtubule-associated protein light chain 3-II (LC3-II) and beclin-1, nevertheless AZA-1-treated hearts evidenced increased levels of the apoptosis markers cleaved caspase-3 and -8, cleaved PARP and Fas ligand. Therefore, while YTX-induced damage to the heart triggers autophagic processes, apoptosis activation occurs in the case of AZA-1. For the first time, activation of cell death signals in cardiomyocytes is demonstrated for these toxins with in vivo experiments, which may be related to alterations of the cardiovascular function. |
| تدمد: | 1432-0738 0340-5761 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b11327c54d07f1455e445a60ecb1cbbTest https://doi.org/10.1007/s00204-016-1862-0Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....7b11327c54d07f1455e445a60ecb1cbb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320738 03405761 |
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