Ginsenoside Rb1 Ameliorates Autophagy of Hypoxia Cardiomyocytes from Neonatal Rats via AMP-Activated Protein Kinase Pathway
| العنوان: | Ginsenoside Rb1 Ameliorates Autophagy of Hypoxia Cardiomyocytes from Neonatal Rats via AMP-Activated Protein Kinase Pathway |
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| المؤلفون: | Sheng-Nan Dai, Hong-Liang Kong, Bo-Han Chen, Shu-mei Zhao, Ai-Jie Hou, Hua-Ting Huang, Xiao-Ming Chen |
| المصدر: | Chinese Journal of Integrative Medicine. 25:521-528 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Ginsenosides, Cell Survival, ATG5, 0211 other engineering and technologies, 02 engineering and technology, AMP-Activated Protein Kinases, 030226 pharmacology & pharmacy, Cathepsin B, 03 medical and health sciences, 0302 clinical medicine, AMP-activated protein kinase, Western blot, Sequestosome-1 Protein, 021105 building & construction, Autophagy, medicine, Animals, Myocytes, Cardiac, Pharmacology (medical), Viability assay, Rats, Wistar, Protein kinase A, biology, medicine.diagnostic_test, Chemistry, Troponin I, AMPK, General Medicine, Adenosine, Molecular biology, Cell Hypoxia, eye diseases, Animals, Newborn, Complementary and alternative medicine, biology.protein, Lysosomes, Biomarkers, Fetal bovine serum, Signal Transduction, medicine.drug |
| الوصف: | To investigate whether ginsenoside-Rb1 (Gs-Rb1) improves the CoCl-induced autophagy of cardiomyocytes via upregulation of adenosine 5′-monophosphate-activated protein kinase (AMPK) pathway. Ventricles from 1- to 3-day-old Wistar rats were sequentially digested, separated and incubated in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum for 3 days followed by synchronization. Neonatal rat cardiomyocytes were randomly divided into 7 groups: control group (normal level oxygen), hypoxia group (500 μmol/L CoCl2), Gs-Rb1 group (200 μmol/L Gs-Rb1 + 500 μmol/L CoCl2), Ara A group (500 μmol/L Ara A + 500 μmol/L CoCl2), Ara A+ Gs-Rb1 group (500 μmol/L Ara A + 200 μmol/L Gs-Rb1 + 500 μmol/L CoCl2), AICAR group [1 mmol/L 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) + 500 μmol/L CoCl2], and AICAR+Gs-Rb1 group (1 mmol/L AICAR + 200 μmol/L Gs-Rb1 + 500 μmol/L CoCl2). Cells were treated for 12 h and cell viability was determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cardiac troponin I (cTnI) levels were detected by enzyme-linked immunosorbent assay (ELISA). AMPK activity was assessed by 2′,7′-dichlorofluorescein diacetate (DCFH-DA) ELISA assay. The protein expressions of Atg4B, Atg5, Atg6, Atg7, microtubule-associated protein 1A/1B-light chain 3 (LC3), P62, and active-cathepsin B were measured by Western blot. Gs-Rb1 significantly improved the cell viability of hypoxia cardiomyocytes (P 0.05). Gs-Rb1 significantly down-regulated P62 levels of hypoxic cardiomyocytes (P |
| تدمد: | 1993-0402 1672-0415 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bd8805654abe02f5dfedc8926308793Test https://doi.org/10.1007/s11655-018-3018-yTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5bd8805654abe02f5dfedc8926308793 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19930402 16720415 |
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