A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice
| العنوان: | A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice |
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| المؤلفون: | Edgar Trujillo, Sergio Rosales-Mendoza, Carlos Angulo |
| المصدر: | Plant Molecular Biology |
| بيانات النشر: | Springer Netherlands, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Multiepitopic, Immunogen, Recombinant protein, Bacterial Toxins, Context (language use), Plant Science, Biology, 01 natural sciences, Epitope, Article, Microbiology, law.invention, 03 medical and health sciences, Epitopes, Mice, Antigen, law, Gene Expression Regulation, Plant, Enteric diseases, Tobacco, Genetics, Animals, Enterotoxigenic Escherichia coli, Plant Proteins, Mice, Inbred BALB C, Mucous Membrane, Vaccines, Edible, Vaccination, food and beverages, General Medicine, Multi epitope, Plants, Genetically Modified, Fusion protein, Antibodies, Bacterial, Recombinant Proteins, Plant-based vaccine, Immunoglobulin A, 030104 developmental biology, Immunoglobulin G, Antibody response, Bacterial Vaccines, Recombinant DNA, Female, Immunization, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | Key message A plant-based multiepitopic protein (LTBentero) containing epitopes from ETEC, S. typhimurium, and V. parahaemolyticus was produced in plants cells and triggered systemic and intestinal humoral responses in immunized mice. Abstract Around 200 million people suffer gastroenteritis daily and more than 2 million people die annually in developing countries due to such pathologies. Vaccination is an alternative to control this global health issue, however new low-cost vaccines are needed to ensure proper vaccine coverage. In this context, plants are attractive hosts for the synthesis and delivery of subunit vaccines. Therefore, in this study a plant-made multiepitopic protein named LTBentero containing epitopes from antigens of enterotoxigenic E. coli, S. typhimurium, and V. parahaemolyticus was produced and found immunogenic in mice. The LTBentero protein was expressed in tobacco plants at up to 5.29 µg g−1 fresh leaf tissue and was deemed immunogenic when administered to BALB/c mice either orally or subcutaneously. The plant-made LTBentero antigen induced specific IgG (systemic) and IgA (mucosal) responses against LTB, ST, and LptD epitopes. In conclusion, multiepitopic LTBentero was functionally produced in plant cells, being capable to trigger systemic and intestinal humoral responses and thus it constitutes a promising oral immunogen candidate in the fight against enteric diseases. |
| اللغة: | English |
| تدمد: | 1573-5028 0167-4412 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80e0e456f3f6ed136e5b884224886628Test http://europepmc.org/articles/PMC7223238Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....80e0e456f3f6ed136e5b884224886628 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735028 01674412 |
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