مؤتمر
In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue
| العنوان: | In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue |
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| المؤلفون: | Chisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, Dmitri B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R., Smith, K. J. |
| بيانات النشر: | Springer Nature Switzerland AG |
| سنة النشر: | 2016 |
| المجموعة: | University College Cork, Ireland: Cork Open Research Archive (CORA) |
| مصطلحات موضوعية: | Brain cortex, Mitochondria, Capillaries, Tension, Pressure, Venules, Surface, Disease, Oxygen, Brain, Vasculature, Confocal microscope |
| الوصف: | Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved ‘halo’ of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function. |
| نوع الوثيقة: | conference object |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-4939-3022-7 978-1-4939-3023-4 1-4939-3022-2 1-4939-3023-0 |
| العلاقة: | Advances in Experimental Medicine and Biology book series (AEMB, volume 876); http://www.isott.org/index.php/news-reader/items/isott-2014-london-uk.htmlTest; https://doi.org/10.1007/978-1-4939-3023-4_66Test; Chisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, D. B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R. and Smith, K. J. (2016) 'In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue', in Elwell, C. E., Leung, T. S. and Harrison, D. K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY, pp. 233-239. doi:10.1007/978-1-4939-3023-4_29; 239; Advances in Experimental Medicine and Biology; 233; http://hdl.handle.net/10468/8857Test; 876 |
| DOI: | 10.1007/978-1-4939-3023-4_29 |
| الإتاحة: | https://doi.org/10.1007/978-1-4939-3023-4_29Test https://doi.org/10.1007/978-1-4939-3023-4_66Test http://hdl.handle.net/10468/8857Test |
| حقوق: | © 2016, the Authors. This chapter was originally published under a CC BY-NC 4.0 license, but has now been made available under a CC BY 4.0 license. ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.490CBF88 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781493930227 9781493930234 1493930222 1493930230 |
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| DOI: | 10.1007/978-1-4939-3023-4_29 |