التفاصيل البيبلوغرافية
| العنوان: |
Creatinine Transport by Basolateral Organic Cation Transporter hOCT2 in the Human Kidney. |
| المؤلفون: |
Yumiko Urakami, Naoko Kimura, Masahiro Okuda, Ken-ichi Inui |
| المصدر: |
Pharmaceutical Research; Jun2004, Vol. 21 Issue 6, p976-981, 6p |
| مصطلحات موضوعية: |
CREATINE, BIOLOGICAL transport, CATIONS, URINE |
| مستخلص: |
Purpose. Creatinine is excreted into urine by tubular secretion in addition to glomerular filtration. The purpose of this study was to clarify molecular mechanisms underlying the tubular secretion of creatinine in the human kidney. Methods. Transport of [14C]creatinine by human organic ion transporters (SLC22A) was assessed by HEK293 cells expressing hOCT1, hOCT2, hOCT2-A, hOAT1, and hOAT3. Results. Among the organic ion transporters examined, only hOCT2 stimulated creatinine uptake when expressed in HEK293 cells. Creatinine uptake by hOCT2 was dependent on the membrane potential. The Michaelis constant (Km) for creatinine transport by hOCT2 was 4.0 mM, suggesting low affinity. Various cationic drugs including cimetidine and trimethoprim, but not anionic drugs, markedly inhibited creatinine uptake by hOCT2. Conclusion. These results suggest that hOCT2, but not hOCT1, is responsible for the basolateral membrane transport of creatinine in the human kidney. [ABSTRACT FROM AUTHOR] |
|
Copyright of Pharmaceutical Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
