Intracellular localization of histone deacetylase HDA6 in plants
| العنوان: | Intracellular localization of histone deacetylase HDA6 in plants |
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| المؤلفون: | Motoaki Seki, Hideyuki Arata, Kazuki Kurita, Yoshiki Habu, Takuya Sakamoto, Sachihiro Matsunaga, Maho Tanaka, Chikako Utsumi, Yoshinori Utsumi, Tomoko M. Matsunaga, Satoshi Takahashi, Jong Myong Kim, Sota Naruse, Yuki Sakamoto, Tetsuya Higashiyama |
| المصدر: | SC10202004090001 NARO成果DBa C10202003260015_9660 |
| بيانات النشر: | Springer Nature, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Manihot, Heterochromatin, Arabidopsis, Plant Science, Histone Deacetylase 6, 01 natural sciences, Plant Roots, Histone Deacetylases, 03 medical and health sciences, Stress, Physiological, Arabidopsis thaliana, Cell Nucleus, biology, Arabidopsis Proteins, Gene Expression Profiling, fungi, food and beverages, Oryza, Histone acetyltransferase, biology.organism_classification, Chromatin, Cell biology, Droughts, 030104 developmental biology, Histone, Acetylation, biology.protein, Histone deacetylase, 010606 plant biology & botany |
| الوصف: | Histone modification is an important epigenetic mechanism in eukaryotes. Histone acetyltransferase and deacetylase regulate histone acetylation levels antagonistically, leading to dynamic control of chromatin structure. One of the histone deacetylases, HDA6, is involved in gene silencing in the heterochromatin regions, chromocenter formation, and metabolic adaptation under drought stress. Although HDA6 plays an important role in chromatin control and response to drought stress, its intracellular localization has not been observed in detail. In this paper, we generated transformants expressing HDA6-GFP in the model plant, Arabidopsis thaliana, and the crops, rice, and cassava. We observed the localization of the fusion protein and showed that HDA6-GFP was expressed in the whole root and localized at the nucleus in Arabidopsis, rice, and cassava. Remarkably, HDA6-GFP clearly formed speckles that were actively colocalized with chromocenters in Arabidopsis root meristem. In contrast, such speckles were unlikely to be formed in rice or cassava. Because AtHDA6 directly binds to the acetate synthesis genes, which function in drought tolerance, we performed live imaging analyses to examine the cellular dynamics of pH in roots and the subnuclear dynamics of AtHDA6 responding to acetic acid treatment. The number of HDA6 speckles increased during drought stress, suggesting a role in contributing to drought stress tolerance. |
| اللغة: | English |
| تدمد: | 1618-0860 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9aeba2f6c29a2fb40be3bd1d59e79376Test http://id.nii.ac.jp/1578/00004958Test/ |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9aeba2f6c29a2fb40be3bd1d59e79376 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16180860 |
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