المؤلفون: Chabattula, Siva Chander, Patra, Bamadeb, Gupta, Piyush Kumar, Govarthanan, Kavitha, Rayala, Suresh Kumar, Chakraborty, Debashis, Verma, Rama Shanker

المصدر: Applied Biochemistry & Biotechnology; Feb2024, Vol. 196 Issue 2, p1058-1078, 21p

مستخلص: Metal/Metal Oxide nanoparticles (M/MO NPs) exhibit potential biomedical applications due to their tunable physicochemical properties. Recently, the biogenic synthesis of M/MO NPs has gained massive attention due to their economical and eco-friendly nature. In the present study, Nyctanthes arbor-tristis (Nat) flower extract-derived Zinc Ferrite NPs (Nat-ZnFe2O4 NPs) were synthesized and physicochemically characterized by FTIR, XRD, FE-SEM, DLS, and other instruments to study their crystallinity, size, shape, net charge, presence of phytocompounds on NP's surface and several other features. The average particle size of Nat-ZnFe2O4 NPs was approx. 25.87 ± 5.67 nm. XRD results showed the crystalline nature of Nat-ZnFe2O4 NPs. The net surface charge on NPs was -13.28 ± 7.18 mV. When tested on mouse fibroblasts and human RBCs, these NPs were biocompatible and hemocompatible. Later, these Nat-ZnFe2O4 NPs exhibited potent anti-neoplastic activity against pancreatic, lung, and cervical cancer cells. In addition, NPs induced apoptosis in tested cancer cells through ROS generation. These in vitro studies confirmed that Nat-ZnFe2O4 NPs could be used for cancer therapy. Moreover, further studies are recommended on ex vivo platforms for future clinical applications. [ABSTRACT FROM AUTHOR]

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المؤلفون: Chabattula, Siva Chander, Gupta, Piyush Kumar, Govarthanan, Kavitha, Varadaraj, Sudha, Rayala, Suresh Kumar, Chakraborty, Debashis, Verma, Rama Shanker

المصدر: Applied Biochemistry & Biotechnology; Jan2024, Vol. 196 Issue 1, p382-399, 18p

مستخلص: Inorganic nanoparticles (NPs) have played an important role as nano-drug delivery systems during cancer therapy in recent years. These NPs can carry cancer therapeutic agents. Due to this, they are considered a promising ancillary to traditional cancer therapies. Among inorganic NPs, Zinc Oxide (ZnO) NPs have been extensively utilized in cellular imaging, gene/drug delivery, anti-microbial, and anti-cancerous applications. In this study, a rapid and cost-effective method was used to synthesize Nat-ZnO NPs using the floral extract of the Nyctanthes arbor-tristis (Nat) plant. Nat-ZnO NPs were physicochemically characterized and tested further on in vitro cancer models. The average hydrodynamic diameter (Zaverage) and the net surface charge of Nat-ZnO NPs were 372.5 ± 70.38 d.nm and −7.03 ± 0.55 mV, respectively. Nat-ZnO NPs exhibited a crystalline nature. HR-TEM analysis showed the triangular shape of NPs. Furthermore, Nat-ZnO NPs were also found to be biocompatible and hemocompatible when tested on mouse fibroblast cells and RBCs. Later, the anti-cancer activity of Nat-ZnO NPs was tested on lung and cervical cancer cells. These NPs displayed potent anti-cancer activity and induced programmed cell death in cancer cells. [ABSTRACT FROM AUTHOR]

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المؤلفون: Padmavathy, Karthika P., Vuttaradhi, Veena Kumari, Venu, Akkanapally, D'Cruze, Lawrence, Saravanan, Roshni, Pitani, Ravishankar, Ganesh, Krishnamurthy, Pacharla, Himavani, Rayala, Suresh Kumar, Prathiba, D., Venkatraman, Ganesh

المصدر: Journal of Molecular Neuroscience; Apr2021, Vol. 71 Issue 4, p724-733, 10p

مستخلص: Malignant astrocytomas presenting in humans of any age group are a challenge to diagnose and treat. Hence, there is a quest for new markers to ascertain their grades and predict disease outcomes. Proline, glutamic acid, and leucine-rich protein 1 (PELP1), a nuclear receptor co-regulator, is an oncogene found in various cancers. We postulate that by screening for PELP1, its correlation with survival outcomes of patients across various grades can indicate a plausible novel diagnostic marker and a potential therapeutic target in gliomas. Immunostaining of 100 cases of astrocytomas for PELP1 was performed on paraffin-embedded sections. Results showed that PELP1 expression increases with higher grades; the mean H-score of PELP1 in grade-I astrocytomas was determined to be 112.3, whereas in grade-IV it was 235.1 (P value = 0.0001). Survival analysis of patients with H-score of 200–300 was only 8.8% and 68.8% in patients with scores of 0–100. PELP1 expression in high-grade astrocytomas is an important factor in determining the outcomes. [ABSTRACT FROM AUTHOR]

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المؤلفون: Mohinudeen, I. A. H. Khwajah, Kanumuri, Rahul, Soujanya, K. N., Shaanker, R. Uma, Rayala, Suresh Kumar, Srivastava, Smita

المصدر: Scientific Reports; 1/14/2021, Vol. 10 Issue 1, p1-11, 11p

مصطلحات موضوعية: CAMPTOTHECIN, ALTERNARIA, ICACINACEAE, ENDOPHYTES

مصطلحات جغرافية: INDIA

مستخلص: Camptothecin the third most in demand alkaloid, is commercially extracted in India from the endangered plant, Nothapodytes nimmoniana. Endophytes, the microorganisms that reside within plants, are reported to have the ability to produce host–plant associated metabolites. Hence, our research aims to establish a sustainable and high camptothecin yielding endophyte, as an alternative source for commercial production of camptothecin. A total of 132 endophytic fungal strains were isolated from different plant parts (leaf, petiole, stem and bark) of N. nimmoniana, out of which 94 were found to produce camptothecin in suspension culture. Alternaria alstroemeriae (NCIM1408) and Alternaria burnsii (NCIM1409) demonstrated camptothecin yields up to 426.7 ± 33.6 µg/g DW and 403.3 ± 41.6 µg/g DW, respectively, the highest reported production to date. Unlike the reported product yield attenuation in endophytes with subculture in axenic state, Alternaria burnsii NCIM1409 could retain and sustain the production of camptothecin up to ~ 200 μg/g even after 12 continuous subculture cycles. The camptothecin biosynthesis in Alternaria burnsii NCIM1409 was confirmed using ¹³C carbon labelling (and cytotoxicity analysis on different cancer cell lines) and this strain can now be used to develop a sustainable bioprocess for in vitro production of camptothecin as an alternative to plant extraction. [ABSTRACT FROM AUTHOR]

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المؤلفون: Perumal, Agilan, Kanumuri, Rahul, Rayala, Suresh Kumar, Nallaiyan, Rajendran

المصدر: Journal of Materials Science; Nov2020, Vol. 55 Issue 32, p15602-15620, 19p, 1 Diagram, 3 Charts, 12 Graphs

مصطلحات موضوعية: NANOCOMPOSITE materials, POLYANILINES, RUTILE, INTERFACIAL bonding, HALLOYSITE, NANOTUBES, CORROSION resistance, CELL adhesion

مستخلص: The long-term permanence of titanium implant was improved by incorporation of polyaniline on TiO2 nanotubes. The polyaniline incorporated TiO2 nanotubes (PANI-2/TNTA) has enhanced the bioactivity and corrosion resistance. Highly ordered nanotubes were formed on the titanium metal with an average tube diameter of ~ 85 nm which was confirmed by HR-SEM. The presence of anatase and rutile mixed phases was identified by XRD analysis. The formation of interfacial bonding between PANI and TNTA was confirmed by Raman and XPS analysis. The lower corrosion current density and higher polarization resistance (Rp) obtained for PANI-2/TNTA nanocomposite revealed the enhanced corrosion resistance behavior in physiological conditions. To ensure the durability of PANI-2/TNTA, the corrosion behavior was analyzed at different applied potentials using dynamic electrochemical impedance spectroscopy (DEIS). Up to 1 V, the higher impedance value was observed in Hanks' solution. The bioactivity of the PANI-2/TNTA nanocomposite was confirmed by the more-notable cell adhesion and proliferation of MG-63 osteoblast-like cells. PANI-2/TNTA has the ability to reduce implant-associated infections. The existence of the amine group is responsible for better biostability and antimicrobial activity. [ABSTRACT FROM AUTHOR]

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المؤلفون: Shakeela, K, Silpa, G, Rayala, Suresh Kumar, Rao, G Ranga

المصدر: Journal of Chemical Sciences; Aug2018, Vol. 130 Issue 8, p1-1, 1p

مصطلحات موضوعية: CAPROLACTAM, POLYOXOMETALATES, CELL lines, COORDINATION compounds, CARCINOGENS

مستخلص: Abstract: Polyoxometalate (POM) based gels are synthesized by reacting POMs with an excess of caprolactam. These POM-entrapped caprolactam gels are thermoreversible and possess good mechanical strength. The gelation of POMs in caprolactam matrix brought the gel acidity similar to the physiological pH. The cytotoxicity studies on SCC 131 cell lines using these gels show 50% of cell death in the range of 2 to 6 mM concentration in 48 h. The supramolecular caprolactam matrix with active protons and the redox property of POMs are responsible for the cytotoxicity effect.Graphical Abstract: SYNOPSIS Two thermoreversible, hydrophilic gels containing Keggin-type polyoxometalates are synthesized by gradually increasing the caprolactam to polyoxometalate mole ratio. They both have physiological pH and show cytotoxicity effect on SCC 131 head and neck cancer cell lines within 24 hours. [ABSTRACT FROM AUTHOR]

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