دورية أكاديمية
Prognostic implications of EGFR protein expression in sporadic colorectal tumors: Correlation with copy number status, mRNA levels and miRNA regulation
| العنوان: | Prognostic implications of EGFR protein expression in sporadic colorectal tumors: Correlation with copy number status, mRNA levels and miRNA regulation |
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| المؤلفون: | Carmen Martínez, Sofía del, Corchete Sánchez, Luis Antonio, Gervas Rios, Ruth, Rodriguez, Alba, Garcia, María, Álcazar, José Antonio, García, Jacinto, Bengoechea, Oscar, Muñoz-Bellvis, Luis, Sayagués Manzano, José María, Abad Hernández, María Mar |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2020 |
| المجموعة: | Universidad de Salamanca: Gredos (Gestión del Repositorio Documental de la Universidad de Salamanca) |
| مصطلحات موضوعية: | Colorrectal tumors, Cancer genomic, Diagnostic-markers, Colonic Neoplasms, neoplasias del colon |
| الوصف: | [EN]Sporadic colorectal cancer (sCRC) is the third most frequent cancer worldwide and the second most common cause of cancer-related deaths (mainly due metastatic dissemination). We investigated the immunohistochemical expression of frequently altered proteins in primary tumors from 51 patients (25 liver metastatic and 26 non-metastatic cases) with a median 103 months follow-up (103 months). We evaluated EGFR copy number (using SNP arrays and FISH) and its expression and regulation (by mRNA and miRNA arrays). We found differences between metastatic and non-metastatic sCRCs for MLH1 (p = 0.05), PMS2 (p = 0.02), CEA (p < 0.001) and EGFR (p < 0.001) expression. EGFR expression was associated with lymph node metastases (p = 0.001), liver metastases at diagnosis (p < 0.001), and advanced stage (p < 0.001). There were associations between EGFR expression-, EGFR gene copy number- and EGFR mRNA levels. We found potential interactions of two miRNAs targeting EGFR expression, (miR-134 and miR-4328, in non-metastatic and metastatic tumors, respectively). EGFR expression was associated with a worse outcome (p = 0.005). Multivariate analysis of prognostic factors for overall survival identified that, the expression of EGFR expression (p = 0.047) and pTNM stage (p < 0.001) predicted an adverse outcome. EGFR expression could be regulated by amplification or polysomies (in metastatic tumors), or miRNAs (miRNA-134, in non-metastatic tumors). EGFR expression in sCRC appears to be related to metastases and poor outcome. ; This work has been partially supported by grants from the Gerencia Regional de Salud de Castilla y León, Valladolid, Spain (GRS2051/A/19), CIBERONC from the ISCIII (CB16/12/00400) and the Fundación Memoria de Don Samuel Solórzano Barruso, Salamanca, Spain |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| العلاقة: | https://www.nature.com/articles/s41598-020-61688-7Test; Del Carmen, S., Corchete, L. A., Gervas, R., Rodriguez, A., Garcia, M., Álcazar, J. A., . & Abad, M. (2020). Prognostic implications of EGFR protein expression in sporadic colorectal tumors: Correlation with copy number status, mRNA levels and miRNA regulation. Scientific Reports, 10(1), 4662. doi:10.1038/s41598-020-61688-7; http://hdl.handle.net/10366/155187Test |
| DOI: | 10.1038/s41598-020-61688-7 |
| الإتاحة: | https://doi.org/10.1038/s41598-020-61688-7Test http://hdl.handle.net/10366/155187Test |
| حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.EA62E52D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-61688-7 |