مورد إلكتروني
First evidence of a retrotransposon-like element in olive (Olea europaea): implications in plant variety identification by SCAR-marker development
| العنوان: | First evidence of a retrotransposon-like element in olive (Olea europaea): implications in plant variety identification by SCAR-marker development |
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| بيانات النشر: | Springer Nature 2001-05 |
| تفاصيل مُضافة: | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Hernández Molina, Pilar Rosa, Raúl de la Rallo, Luis Martín, Antonio Dorado, Gabriel |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | When developing SCARs by sequencing RAPD markers useful for olive variety identification, one RAPD sequence of 407 bp has been identified that shows significant DNA homology with more than 160 retrotransposon-like sequences. A generally coherent phylogenetic tree has been constructed based on the homologous retrotransposon-like sequences, reflecting genetic distances in 35 species belonging to eight kingdoms. The implications of this finding in the development of SCAR markers are discussed. In addition, specific oligonucleotide primers have been developed to amplify the DNA region and have a practical application as an internal amplification control in SCAR-based multiplexed PCRs. To our knowledge, this is the first report of a retrotransposon-like element in olive. |
| مصطلحات الفهرس: | Kingdom and biological evolution, Phylogenetic tree, RAPD-PCR, Retrotransposon-like sequence, SCAR, artículo |
| URL: | Sí |
| الإتاحة: | Open access content. Open access content closedAccess |
| ملاحظة: | English |
| أرقام أخرى: | CTK oai:digital.csic.es:10261/184284 Theoretical and applied genetics 102(6-79: 1082- 1087 (2001) 0040-5752 10.1007/s001220000515 1432-2242 1257720850 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1257720850 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |