Integrative Structure Determination of Protein Assemblies by Satisfaction of Spatial Restraints
| العنوان: | Integrative Structure Determination of Protein Assemblies by Satisfaction of Spatial Restraints |
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| المؤلفون: | Frank Alber, Brian T. Chait, Michael P. Rout, Andrej Sali |
| المصدر: | Computational Biology ISBN: 9781848001244 Protein-protein Interactions and Networks |
| بيانات النشر: | Springer London, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Macromolecular assembly, Structure (mathematical logic), business.industry, Component (UML), Completeness (order theory), Experimental data, Structural engineering, Function (mathematics), business, Biological system, Representation (mathematics), Spatial analysis, Mathematics |
| الوصف: | To understand the cell, we need to determine the structures of macromolecular assemblies, many of which consist of tens to hundreds of components. A great variety of experimental data can be used to characterize the assemblies at several levels of resolution, from atomic structures to component configurations. To maximize completeness, resolution, accuracy, precision and efficiency of the structure determination, a computational approach is needed that can use spatial information from a variety of experimental methods. We propose such an approach, defined by its three main components: a hierarchical representation of the assembly, a scoring function consisting of spatial restraints derived from experimental data, and an optimization method that generates structures consistent with the data. We illustrate the approach by determining the configuration of the 456 proteins in the nuclear pore complex from Baker’s yeast. |
| ردمك: | 978-1-84800-124-4 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::73bfba486c785d81303e1582e2be36e5Test https://doi.org/10.1007/978-1-84800-125-1_6Test |
| رقم الانضمام: | edsair.doi...........73bfba486c785d81303e1582e2be36e5 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781848001244 |
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