Both Bupivacaine and Levobupivacaine inhibit colon cancer cell growth but not melanoma cells in vitro
| العنوان: | Both Bupivacaine and Levobupivacaine inhibit colon cancer cell growth but not melanoma cells in vitro |
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| المؤلفون: | Lin Chen, Lingzhi Wu, Kazuyoshi Hirota, Hailin Zhao, Chongfang Han, Joe Masters, Daqing Ma, Tianci Li |
| المصدر: | Journal of Anesthesia |
| بيانات النشر: | Springer Japan, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | GRP78, Surgical stress, Colorectal cancer, SURGERY, Apoptosis, CHOP, ANESTHETIC TECHNIQUE, PATHWAY, 03 medical and health sciences, 0302 clinical medicine, ENDOPLASMIC-RETICULUM STRESS, 030202 anesthesiology, Anesthesiology, ABDOMINIS PLANE BLOCK, Cell Line, Tumor, Medicine, BREAST-CANCER, Humans, Anesthetics, Local, RECURRENCE, Melanoma, Endoplasmic Reticulum Chaperone BiP, Levobupivacaine, Cell Proliferation, Bupivacaine, Science & Technology, business.industry, Cell growth, UNFOLDED PROTEIN RESPONSE, 030208 emergency & critical care medicine, medicine.disease, Colon cancer, Anesthesiology and Pain Medicine, ROPIVACAINE, Anesthesia, Colonic Neoplasms, Cancer research, Original Article, business, Life Sciences & Biomedicine, medicine.drug |
| الوصف: | Background Retrospective studies indicate that the use of regional anaesthesia causes a reduction in cancer recurrence after oncological surgery, which could be due to anaesthetic’s negating effect on immunosuppression related to the surgical stress response. Local anaesthetics may also exert direct suppressive effects on malignant cells, an area where further investigation is urgently needed. Methods Human colon cancer cells and human melanoma cells were cultured and then treated with 1 mM bupivacaine or levobupivacaine for up to 24 or 48 h. Their migratory ability was measured by scratch assay, proliferation determined with Ki67 immunofluorescence staining, and apoptosis accessed with annexin V and PI staining on flow cytometry. The effects of bupivacaine and levobupivacaine on cellular signaling and molecular response, specifically, on endoplasmic reticulum stress (ERS), were studied with immunostaining and western blot. Results In colon cancer cells, treatment with bupivacaine and levobupivacaine significantly inhibited cell migration (**p |
| اللغة: | English |
| تدمد: | 1438-8359 0913-8668 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::271fe3002970c602c1e0d4808c4022caTest http://europepmc.org/articles/PMC6373235Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....271fe3002970c602c1e0d4808c4022ca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14388359 09138668 |
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