دورية أكاديمية
Electron Spray Ionization Mass Spectrometry And 2D P-31 Nmr For Monitoring O-18/O-16 Isotope Exchange And Turnover Rates Of Metabolic Oligophosphates
| العنوان: | Electron Spray Ionization Mass Spectrometry And 2D P-31 Nmr For Monitoring O-18/O-16 Isotope Exchange And Turnover Rates Of Metabolic Oligophosphates |
|---|---|
| المؤلفون: | Nemutlu, Emirhan, Juranic, Nenad, Zhang, Song, Ward, Lawrence E., Dutta, Tumpa, Nair, K. Sreekumaran, Terzic, Andre, Macura, Slobodan, Dzeja, Petras P. |
| المساهمون: | Kimya |
| بيانات النشر: | Springer Heidelberg |
| سنة النشر: | 2012 |
| المجموعة: | Hacettepe University Institutional Repository |
| مصطلحات موضوعية: | Biochemistry & Molecular Biology, Chemistry |
| الوصف: | A new method was here developed for the determination of O-18-labeling ratios in metabolic oligophosphates, such as ATP, at different phosphoryl moieties (alpha-, beta-, and gamma-ATP) using sensitive and rapid electrospray ionization mass spectrometry (ESI-MS). The ESI-MS-based method for monitoring of O-18/O-16 exchange was validated with gas chromatography-mass spectrometry and 2D P-31 NMR correlation spectroscopy, the current standard methods in labeling studies. Significant correlation was found between isotopomer selective 2D P-31 NMR spectroscopy and isotopomer less selective ESI-MS method. Results demonstrate that ESI-MS provides a robust analytical platform for simultaneous determination of levels, O-18-labeling kinetics and turnover rates of alpha-, beta-, and gamma-phosphoryls in ATP molecule. Such method is advantageous for large scale dynamic phosphometabolomic profiling of metabolic networks and acquiring information on the status of probed cellular energetic system. |
| نوع الوثيقة: | article in journal/newspaper conference object |
| وصف الملف: | text/plain; application/pdf |
| اللغة: | English |
| تدمد: | 1618-2642 |
| العلاقة: | Analytical And Bioanalytical Chemistry; https://doi.org/10.1007/s00216-012-5899-5Test; http://hdl.handle.net/11655/19622Test; 403; 697; 706 |
| DOI: | 10.1007/s00216-012-5899-5 |
| الإتاحة: | https://doi.org/10.1007/s00216-012-5899-5Test http://hdl.handle.net/11655/19622Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.C11643F5 |
| قاعدة البيانات: | BASE |
| تدمد: | 16182642 |
|---|---|
| DOI: | 10.1007/s00216-012-5899-5 |