دورية أكاديمية
No evidence of enteroviruses in the intestine of patients with type 1 diabetes
| العنوان: | No evidence of enteroviruses in the intestine of patients with type 1 diabetes |
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| المؤلفون: | Mercalli, A., Lampasona, V., Klingel, K., Albarello, L., Lombardoni, C., Ekström, Johanna, Sordi, V., Bolla, A., Mariani, A., Bzhalava, Davit, Dillner, Joakim, Roivainen, M., Bosi, E., Piemonti, L. |
| المصدر: | Diabetologia; 55(9), pp 2479-2488 (2012) ; ISSN: 1432-0428 |
| بيانات النشر: | Springer |
| سنة النشر: | 2012 |
| المجموعة: | Lund University Publications (LUP) |
| مصطلحات موضوعية: | Endocrinology and Diabetes, Enterovirus, Intestine, Type 1 diabetes |
| الوصف: | The purpose of this study was to investigate whether the gut mucosa is a reservoir for enterovirus persistence in patients with type 1 diabetes. Small intestine biopsy samples from 25 individuals at different stages of type 1 diabetes, 21 control individuals and 27 individuals with coeliac disease were analysed for the presence of enterovirus RNA by using both radioactive in-situ hybridisation and real-time RT-PCR and for the presence of enterovirus proteins by immunostaining with antibodies against VP1 and VP4-2-3 capsid proteins and virus polymerase. Lymphocytic enteropathy and serum anti-VP1 antibodies were also evaluated at the time of biopsy. Moreover, high-throughput sequencing was performed to identify viral transcripts or genomes. Enterovirus was not detected by in-situ hybridisation or RT-PCR in any of the individuals tested. Immunohistology revealed a few stained cells in the intestinal epithelium in a low number of individuals, with no difference between diabetic and non-diabetic individuals. Levels of serum IgG against VP1 did not differ between control individuals and those with diabetes or coeliac disease and no evidence of diabetes-related lymphocytic enteropathy was detected. High-throughput sequencing did not reveal specific enterovirus sequences in the gut mucosa of individuals with type 1 diabetes. Prolonged/persistent enterovirus infections in gut mucosa are not common in patients with type 1 diabetes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://lup.lub.lu.se/record/3055707Test; http://dx.doi.org/10.1007/s00125-012-2591-4Test; wos:000307301800024; scopus:84866122387; pmid:22684312 |
| DOI: | 10.1007/s00125-012-2591-4 |
| الإتاحة: | https://doi.org/10.1007/s00125-012-2591-4Test https://lup.lub.lu.se/record/3055707Test |
| رقم الانضمام: | edsbas.A4BC4BAC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00125-012-2591-4 |
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