Engineering of the baculovirus expression system for optimized protein production
| العنوان: | Engineering of the baculovirus expression system for optimized protein production |
|---|---|
| المؤلفون: | Alexandra Marisa Targovnik, María Martínez-Solís, Salvador Herrero |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Springer, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Glycosylation, PROTEIN EXPRESSION, Computational biology, Genome, Viral, INGENIERÍAS Y TECNOLOGÍAS, Biology, Protein Engineering, Applied Microbiology and Biotechnology, Protein expression, law.invention, Cell Line, Biotecnología Industrial, 03 medical and health sciences, chemistry.chemical_compound, Industrial Microbiology, INSECT CELLS, law, Protein biosynthesis, Animals, Vector (molecular biology), NUCLEOPOLYHEDROVIRUS, INSECT BIOFACTORY, 030304 developmental biology, Mammals, 0303 health sciences, Insect cell, 030306 microbiology, Baculovirus expression, purl.org/becyt/ford/2.9 [https], General Medicine, Recombinant Proteins, chemistry, purl.org/becyt/ford/2 [https], Recombinant DNA, Microorganisms, Genetically-Modified, Baculoviridae, DNA, Biotechnology |
| الوصف: | Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multipleinsect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vectorfor the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinantprotein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for thegeneration of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins.In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses andfuture developments with respect to the main aspects involved in protein production using the BEVS. Fil: Martínez Solís, María. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España Fil: Herrero, Salvador. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España Fil: Targovnik, Alexandra Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Universidad de Valencia. Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina; España |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cc787319df1fe99e69d41ddab18cfc6Test http://link.springer.com/10.1007/s00253-018-9474-7Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6cc787319df1fe99e69d41ddab18cfc6 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |