-
1دورية أكاديمية
المؤلفون: Lee, JS Lee, Jae-Seong, Ha, TK Ha, Tae-Kwang, Lee, SJ Lee, Seung-Joo, Lee, GM Lee, Gyun-Min
مصطلحات موضوعية: HAMSTER OVARY CELLS, RECOMBINANT-HUMAN-ERYTHROPOIETIN, N-GLYCOLYLNEURAMINIC ACID, EXPRESSING CHO-CELLS, ENHANCED SIALYLATION, CULTURE-CONDITIONS, SIALIC-ACID, SODIUM-BUTYRATE, PICHIA-PASTORIS, GLYCOSYLATION
الوصف: Erythropoietin is a major regulator of erythropoiesis which maintains the body's red blood cell mass and tissue oxygenation at an optimum level. Recombinant human erythropoietin (rhEPO), which is a widely used therapeutic agent for the treatment of anemia and which represents one of the largest biopharmaceuticals markets, is produced from recombinant Chinese hamster ovary cells. rhEPO is a glycoprotein with complex glycan structure, which is responsible for its therapeutic efficacy, including the in vivo activity and half-life. In order to obtain an optimal and consistent glycoform profile of rhEPO and concurrently maintain a high production yield, various approaches in drug development and cell culture technology have been attempted. Recent advances in rhEPO production are classified into three types: the development of improved rhEPO molecules by protein engineering; improvement of production host cells by genetic engineering; and culture condition optimization by fine control of the production mode/system, process parameters, and culture media. In this review, we focus on rhEPO production strategies as they have progressed thus far. Furthermore, the current status of the market and outlook on rhEPO and its derivatives are discussed.
-
2
المؤلفون: Goto, Kohta, Fukuda, Kenji, 福田, 健二, Senda, Akitsugu, Saito, Tadao, 齋藤, 忠夫, Kimura, Kazumasa, Glander, Kenneth E., Hinde, Katie, Dittus, Wolfgang, Milligan, Lauren A., Power, Michael L., Oftedal, Olav T., Urashima, Tadasu, 浦島, 匡
مصطلحات موضوعية: old world monkey, new world monkey, milk oligosaccharide, rhesus macaque, toque macaque, baboon, capuchin, mantled howler, squirrel monkey, N-glycolylneuraminic acid
الوصف: application/pdf ; Human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. Neutral and acidic oligosaccharides were isolated from the milk of three species of Old World or catarrhine monkeys （Cercopithecidae: rhesus macaque （Macaca mulatta), toque macaque （Macaca sinica) and Hamadryas baboon （Papio hamadryas)) and three of New World or platyrrhine monkeys （Cebidae: tufted capuchin （Cebus apella)and Bolivian squirrel monkey （Saimiri boliviensis); Atelidae: mantled howler （Alouatta palliata)). The milks of these species contained 6-8% total sugar, most of which was lactose: the estimated ratio of oligosaccharides to lactose in Old World monkeys （1:4 to 1:6) was greater than in New World monkeys （1:12 to 1:23). The chemical structures of the oligosaccharides were determined mainly by 1H-NMR spectroscopy. Oligosaccharides containing the type II unit （Gal（β1-4)GlcNAc) were found in the milk of the rhesus macaque, toque macaque, Hamadryas baboon and tufted capuchin, but oligosaccharides containing the type I unit （Gal（β1-3)GlcNAc), which have been found in human and many great ape milks, were absent from the milk of all species studied. Oligosaccharides containing Lewis x （Gal（β1-4)[Fuc（α1-3)]GlcNAc) and 3-fucosyl lactose （Gal（β1-4)[Fuc（α1-3)]Glc) were found in the milk of the three cercopithecid monkey species, while 2-fucosyl lactose （Fuc（α1-2)Gal（β1-4)Glc) was absent from all species studied. All of these milks contained acidic oligosaccharides that had N-acetylneuraminic acid as part of their structures, but did not contain oligosaccharides that had N-glycolylneuraminic acid, in contrast to the milk or colostrum of great apes which contain both types of acidic oligosaccharides. Two GalNAc-containing oligosaccharides, lactose 3’-O-sulfate and lacto-N-novopentaose I （Gal（β1-3)[Gal（β1-4)GlcNAc（β1-6)]Gal（β1-4)Glc) were found only in the milk of rhesus macaque, hamadryas baboon and tufted capuchin, ...
وصف الملف: application/pdf
العلاقة: Glycoconjugate Journal; 27; 703; 715; AA10451191; https://obihiro.repo.nii.ac.jp/record/731/files/GLYC594.pdfTest
