دورية أكاديمية
Quantitative assessment of natural toxicity in sponges: toxicity bioassay versus compound quantification
| العنوان: | Quantitative assessment of natural toxicity in sponges: toxicity bioassay versus compound quantification |
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| المؤلفون: | Martí, Ruth, Fontana, A., Uriz, María Jesús, Cimino, C. |
| بيانات النشر: | Springer |
| سنة النشر: | 2003 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Dysidea avara, Ircinia variabilis, Natural toxicity, Sesterpene, Avarol, Palinurin, Secondary metabolites, Crude extracts, Bioactivity, Bioassays, Microtox, Chemical quantification, Sponges |
| الوصف: | Este artículo contiene 12 páginas, 4 figuras,1 tabla. ; Microtox assay was used to assess the natural toxicity of two sponges, Dysidea avara and Ircinia variabilis. The activity of crude extracts and major metabolites were compared. Methanol extract of D. avara was more toxic than that of acetone and was as toxic as pure avarol, thus suggesting that the toxicity of the sponge was mainly due to this metabolite. We also quantified palinurin, the major metabolite of I. variabilis, in specimens from several habitats. With the same methanol extracts used for palinurin quantification, we ran the Microtox assay and found a positive significant regression between toxicity and concentration of this metabolite. Pure palinurin was tested at the same concentration present in the extract, and the toxicity recorded was higher than that of the methanol extract. As with avarol from D. avara, palinurin is the main secondary metabolite that confers toxicity to I. variabilis. The results confirm that the standardized Microtox assay is an accurate and reproducible tool for assessing the toxicity of crude extracts and pure metabolites of marine organisms. These results also suggest that methanol is more suitable than acetone for the detection of species toxicity by Microtox. The method is faster and easier to perform than chemical quantification even when the sponge chemistry is known, and is appropriate for studies on variation in natural toxicity over a range of environmental conditions. ; This research was supported by bilateral CSIC/CNR 2001–2002 to AF and MJU and CICYT REN2001-2312-CO3/MAR grants. It also benefited from a Government of Catalonia fellowship to R. Martí ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0098-0331 1573-1561 |
| العلاقة: | https://link.springer.com/article/10.1023/A:1024201100811Test; Sí; Journal of Chemical Ecology 29(6):1307-1318 (2003); http://hdl.handle.net/10261/159868Test |
| الإتاحة: | http://hdl.handle.net/10261/159868Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.FB5ED18D |
| قاعدة البيانات: | BASE |
| تدمد: | 00980331 15731561 |
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