دورية أكاديمية
Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics
| العنوان: | Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics |
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| المؤلفون: | White, Matthew E H, Sinn, Ludwig R, Jones, D Marc, de Folter, Joost, Aulakh, Simran Kaur, Wang, Ziyue, Flynn, Helen R, Krüger, Lynn, Tober-Lau, Pinkus, Demichev, Vadim, Kurth, Florian, Mülleder, Michael, Blanchard, Véronique, Messner, Christoph B, Ralser, Markus |
| المصدر: | White, Matthew E H; Sinn, Ludwig R; Jones, D Marc; de Folter, Joost; Aulakh, Simran Kaur; Wang, Ziyue; Flynn, Helen R; Krüger, Lynn; Tober-Lau, Pinkus; Demichev, Vadim; Kurth, Florian; Mülleder, Michael; Blanchard, Véronique; Messner, Christoph B; Ralser, Markus (2023). Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics. Nature Biomedical Engineering, 8(3):233-247. |
| بيانات النشر: | Springer |
| سنة النشر: | 2023 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Swiss Institute of Allergy and Asthma Research, 610 Medicine & health, Computer Science Applications, Biomedical Engineering, Medicine (miscellaneous), Bioengineering, Biotechnology |
| الوصف: | Protein glycosylation, a complex and heterogeneous post-translational modification that is frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a data-independent acquisition technique for the large-scale mass-spectrometric quantification of glycopeptides in plasma samples. The technique, which we named ‘OxoScan-MS’, identifies oxonium ions as glycopeptide fragments and exploits a sliding-quadrupole dimension to generate comprehensive and untargeted oxonium ion maps of precursor masses assigned to fragment ions from non-enriched plasma samples. By applying OxoScan-MS to quantify 1,002 glycopeptide features in the plasma glycoproteomes from patients with COVID-19 and healthy controls, we found that severe COVID-19 induces differential glycosylation in IgA, haptoglobin, transferrin and other disease-relevant plasma glycoproteins. OxoScan-MS may allow for the quantitative mapping of glycoproteomes at the scale of hundreds to thousands of samples. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2157-846X |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/252756/1/ZORA_s41551_023_01067_5.pdfTest; info:pmid/37474612; urn:issn:2157-846X |
| DOI: | 10.5167/uzh-252756 |
| DOI: | 10.1038/s41551-023-01067-5 |
| الإتاحة: | https://doi.org/10.5167/uzh-25275610.1038/s41551-023-01067-5Test https://www.zora.uzh.ch/id/eprint/252756Test/ https://www.zora.uzh.ch/id/eprint/252756/1/ZORA_s41551_023_01067_5.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.8FFFC6B4 |
| قاعدة البيانات: | BASE |
| تدمد: | 2157846X |
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| DOI: | 10.5167/uzh-252756 |