دورية أكاديمية
Stem Cell Reports Resource Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
| العنوان: | Stem Cell Reports Resource Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells |
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| المؤلفون: | Santos, Renata, Vadodaria, Krishna, C, Jaeger, Baptiste, N, Mei, Arianna, Lefcochilos-Fogelquist, Sabrina, Mendes, Ana P.D., Erikson, Galina, Shokhirev, Maxim, Randolph-Moore, Lynne, Fredlender, Callie, Dave, Sonia, Oefner, Ruth, Fitzpatrick, Conor, Pena, Monique, Barron, Jerika, J, Ku, Manching, Denli, Ahmet, M, Kerman, Bilal, E, Charnay, Patrick, Kelsoe, John, R., Marchetto, Maria, C, Gage, Fred, H |
| المساهمون: | Institut de psychiatrie et neurosciences de Paris (IPNP - U1266 Inserm - Paris Descartes), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Psychiatry, University of California San Diego (UC San Diego), University of California (UC)-University of California (UC), The Salk Institute for Biological Studies |
| المصدر: | ISSN: 2198-7866 ; Current Stem Cell Reports ; https://hal.science/hal-02328326Test ; Current Stem Cell Reports, 2017, 8 (6), pp.1757-1769. ⟨10.1016/j.stemcr.2017.05.011⟩. |
| بيانات النشر: | HAL CCSD Springer |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Astrocyte dysfunction and neuroinflammation are detrimental features in multiple pathologies of the CNS. Therefore, the development of methods that produce functional human astrocytes represents an advance in the study of neurological diseases. Here we report an efficient method for inflammation-responsive astrocyte generation from induced pluripotent stem cells (iPSCs) and embryonic stem cells. This protocol uses an intermediate glial progenitor stage and generates functional astrocytes that show levels of glutamate uptake and calcium activation comparable with those observed in human primary astrocytes. Stimulation of stem cell-derived astrocytes with interleukin-1b or tumor necrosis factor a elicits a strong and rapid pro-inflammatory response. RNA-sequencing transcriptome profiling confirmed that similar gene expression changes occurred in iPSC-derived and primary astrocytes upon stimulation with interleukin-1b. This protocol represents an important tool for modeling in-a-dish neuro-logical diseases with an inflammatory component, allowing for the investigation of the role of diseased astrocytes in neuronal degeneration. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-02328326; https://hal.science/hal-02328326Test; https://hal.science/hal-02328326/documentTest; https://hal.science/hal-02328326/file/Santos%20et%20al%20SCR%202017.pdfTest |
| DOI: | 10.1016/j.stemcr.2017.05.011 |
| الإتاحة: | https://doi.org/10.1016/j.stemcr.2017.05.011Test https://hal.science/hal-02328326Test https://hal.science/hal-02328326/documentTest https://hal.science/hal-02328326/file/Santos%20et%20al%20SCR%202017.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-nc-ndTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.CECFD65B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.stemcr.2017.05.011 |
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