التفاصيل البيبلوغرافية
| العنوان: |
The cytoprotective capacity of processed human cardiac extracellular matrix |
| المؤلفون: |
Kappler, Benjamin, Anic, Petra, Becker, Matthias, Bader, Andreas, Klose, Kristin, Klein, Oliver, Oberwallner, Barbara, Choi, Yeong-Hoon, Falk, Volkmar, Stamm, Christof |
| بيانات النشر: |
SPRINGER |
| سنة النشر: |
2016 |
| المجموعة: |
Cologne University: KUPS |
| مصطلحات موضوعية: |
ddc:no |
| الوصف: |
Freshly isolated human cardiac extracellular matrix sheets (cECM) have been shown to support stem cell proliferation and tissue-specific lineage commitment. We now developed a protocol for standardized production of durable, bio-functional hcECM microparticles and corresponding hydrogel, and tested its cytoprotective effects on contractile cells subjected to ischemia-like conditions. Human ventricular myocardium was decellularized by a 3-step protocol, including Tris/EDTA, SDS and serum incubation (cECM). Following snap-freezing and lyophilization, microparticles were created and characterized by laser diffraction, dynamic image analysis (DIA), and mass spectrometry. Moreover, cECM hydrogel was produced by pepsin digestion. Baseline cell-support characteristics were determined using murine HL-1 cardiomyocytes, and the cytoprotective effects of ECM products were tested under hypoxia and glucose/serum deprivation. In cECM, glycoproteins (thrombospondin 1, fibronectin, collagens and nidogen-1) and proteoglycans (dermatopontin, lumican and mimecan) were preserved, but residual intracellular and blood-borne proteins were also detected. The median particle feret diameter was 66 mu m (15-157 mu m) by laser diffraction, and 57 mu m (20-182 mu m) by DIA with crystal violet staining. HL-1 cells displayed enhanced metabolic activity (39 +/- 12 %, P < 0.05) and proliferation (16 +/- 3 %, P < 0.05) when grown on cECM microparticles in normoxia. During simulated ischemia, cECM microparticles exerted distinct cytoprotective effects (MTS conversion, 240 +/- 32 %; BrdU uptake, 45 +/- 14 %; LDH release, -72 +/- 7 %; P < 0.01, each). When cECM microparticles were solubilized to form a hydrogel, the cytoprotective effect was initially abolished. However, modifying the preparation process (pepsin digestion at pH 2 and 25 degrees C, 1 mg/ml final cECM concentration) restored the cytoprotective cECM activity. Extracellular matrix from human myocardium can be processed to yield standardized durable microparticles that exert ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
Kappler, Benjamin, Anic, Petra, Becker, Matthias, Bader, Andreas, Klose, Kristin, Klein, Oliver, Oberwallner, Barbara, Choi, Yeong-Hoon, Falk, Volkmar orcid:0000-0002-7911-8620 and Stamm, Christof (2016). The cytoprotective capacity of processed human cardiac extracellular matrix. J. Mater. Sci.-Mater. Med., 27 (7). DORDRECHT: SPRINGER. ISSN 1573-4838 |
| الإتاحة: |
https://kups.ub.uni-koeln.de/27094Test/ |
| رقم الانضمام: |
edsbas.2E8A7CEC |
| قاعدة البيانات: |
BASE |
