Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins
| العنوان: | Tumor biology of non-metastatic stages of clear cell renal cell carcinoma; overexpression of stearoyl desaturase-1, EPO/EPO-R system and hypoxia-related proteins |
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| المؤلفون: | Nora Cristina Brandan, María Victoria Aguirre, Adriana M. Torres, Juan Santiago Todaro, Tania Romina Stoyanoff, Juan Pablo Rodríguez, Juan Pablo Melana Colavita, Joaquín Espada |
| المصدر: | Tumor Biology, 2016, vol. 37, no. 10, p. 1-13. Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) Universidad Nacional del Nordeste instacron:UNNE CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Springer, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, 0301 basic medicine, Pathology, Erythropoietin (epo), Apoptosis, Immunoenzyme Techniques, Epo Receptor, purl.org/becyt/ford/1 [https], chemistry.chemical_compound, 0302 clinical medicine, hemic and lymphatic diseases, Epo receptor (epo-r), Receptors, Erythropoietin, Tumor Cells, Cultured, Clear Renal Cell Carcinoma, Aged, 80 and over, Reverse Transcriptase Polymerase Chain Reaction, General Medicine, Middle Aged, Prognosis, Kidney Neoplasms, Survival Rate, Vascular endothelial growth factor, Vascular endothelial growth factor A, 030220 oncology & carcinogenesis, Immunohistochemistry, Female, Stearoyl Desaturase -1, Stearoyl-CoA Desaturase, medicine.drug, Adult, medicine.medical_specialty, Blotting, Western, Biology, Real-Time Polymerase Chain Reaction, Young Adult, 03 medical and health sciences, Biomarkers, Tumor, medicine, Humans, RNA, Messenger, purl.org/becyt/ford/1.6 [https], Carcinoma, Renal Cell, Erythropoietin, Aged, Cell Proliferation, Neoplasm Staging, Cancer, Kinase insert domain receptor, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Vascular Endothelial Growth Factor Receptor-2, Clear cell renal cell carcinoma, 030104 developmental biology, Clear cell renal cell carcinoma (ccrcc), chemistry, Tumor progression, Neoplasm Grading, Stearoyl desaturase-1 (scd-1) |
| الوصف: | Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal carcinomas. There is great interest to know the molecular basis of the tumor biology of ccRCC that might contribute to a better understanding of the aggressive biological behavior of this cancer and to identify early biomarkers of disease. This study describes the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (hypoxia-inducible factor (HIF)-1α, erythropoietin (EPO), vascular endothelial growth factor (VEGF)), their receptors (EPO-R, VEGFR-2), and stearoyl desaturase-1 (SCD-1) in early stages of ccRCC. Tissue samples were obtained at the Urology Unit of the J.R. Vidal Hospital (Corrientes, Argentina), from patients who underwent radical nephrectomy for renal cancer between 2011 and 2014. Four experimental groups according to pathological stage and nuclear grade were organized: T1G1 (n = 6), T2G1 (n = 4), T1G2 (n = 7), and T2G2 (n = 7). The expression of HIF-1α, EPO, EPO-R, VEGF, VEGFR-2, Bcl-xL, and SCD-1 were evaluated by immunohistochemistry, Western blotting, and/or RT-PCR. Apoptosis was assessed by the TUNEL in situ assay, and tumor proliferation was determined by Ki-67 immunohistochemistry. Data revealed that HIF-1α, EPO, EPO-R, VEGF, and VEGF-R2 were overexpressed in most samples. The T1G1 group showed the highest EPO levels, approximately 200 % compared with distal renal tissue. Bcl-xL overexpression was concomitant with the enhancement of proliferative indexes. SCD-1 expression increased with the tumor size and nuclear grade. Moreover, the direct correlations observed between SCD-1/HIF-1α and SCD-1/Ki-67 increments suggest a link among these molecules, which would determine tumor progression in early stages of ccRCC. Our results demonstrate the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (HIF-1α, EPO, VEGF), their receptors (EPO-R, VEGFR-2), and SCD-1 in early stages of ccRCC. Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Todaro, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina Fil: Espada, Joaquin Diego. Hospital Vidal. Dpto. de Urología. Corrientes; Argentina Fil: Melana Colavita, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina Fil: Torres, Adriana Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Aguirre, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c17d88298d1461862455ef9fa00dd53Test http://repositorio.unne.edu.ar/handle/123456789/28104Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0c17d88298d1461862455ef9fa00dd53 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |