التفاصيل البيبلوغرافية
| العنوان: |
High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas. |
| المؤلفون: |
Tomas Alanentalo, Christina E. Lore´n, A˚sa Larefalk, James Sharpe, Dan Holmberg, Ulf Ahlgren |
| المصدر: |
Journal of Biomedical Optics. Sep2008, Vol. 13 Issue 5, p54070-54070. 1p. |
| مصطلحات موضوعية: |
*DIAGNOSIS of endocrine diseases, *THREE-dimensional imaging, *DIABETES, *LABORATORY mice, *INSULIN, *OPTICAL tomography |
| مستخلص: |
A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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