Extracts of Koelreuteria henryi Dummer induce apoptosis and autophagy by inhibiting dihydrodiol dehydrogenase, thus enhancing anticancer effects
| العنوان: | Extracts of Koelreuteria henryi Dummer induce apoptosis and autophagy by inhibiting dihydrodiol dehydrogenase, thus enhancing anticancer effects |
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| المؤلفون: | Hui Yu Yang, Kuan-Chih Chow, Cheng Lin Yang, Shiow-Her Chiou, Janaki N. Sudhakar, Shu-Fen Chiang, Shu Li Wang, Ching Kuo Lee, Hsiu Ching Yang, Chih Yi Chen, Chien Min Chen, Yung Yen Chiang, Shwn-Huey Shieh, Hsin Yuan Fang, Hsiu Wen Huang |
| المصدر: | International Journal of Molecular Medicine. 32:577-584 |
| بيانات النشر: | Spandidos Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | DNA repair, Antineoplastic Agents, Apoptosis, Biology, Ceramides, Sapindaceae, Downregulation and upregulation, Cell Line, Tumor, Autophagy, Genetics, Humans, Cytotoxic T cell, Gene silencing, Enzyme Inhibitors, Kaempferols, chemistry.chemical_classification, Plant Extracts, Cell Membrane, Drug Synergism, General Medicine, Enzyme, chemistry, Cancer cell, Cancer research, Oxidoreductases |
| الوصف: | Dihydrodiol dehydrogenase (DDH) is frequently detected in cancer cells, and its overexpression correlates with drug resistance, the downregulation of DNA repair mechanisms, increased frequency of tumor recurrence, cancer cell metastasis and poor prognosis. The silencing of DDH expression using siRNA, on the other hand, reduces drug resistance and cancer cell mobility. These data suggest that DDH may be an oncogene-related protein. However, no specific DDH inhibitor has been identified to date. Thus, in this study, we used DDH as a target enzyme in a live-cell enzyme-linked immunosorbent assay to screen Chinese medicinal herb extracts (CMHEs) with the aim of identifying a DDH inhibitor. Using this method, we found 49 among 796 CMHEs that inhibited DDH expression. We selected three potential extracts, which had the highest activity against DDH, for further fractionation using high-performance liquid chromatography. The active ingredient was identified by immunoblot analysis. The function of the active ingredient was characterized by cell function analysis. Our results revealed that the CMHE-purified compounds targeted DDH, inducing autophagy and reducing DNA repair, which in turn enhanced the cytotoxic effects of the anticancer drugs and irradiation. |
| تدمد: | 1791-244X 1107-3756 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db5beb83f12d7f3c8153c427ebe5d5ebTest https://doi.org/10.3892/ijmm.2013.1441Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....db5beb83f12d7f3c8153c427ebe5d5eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1791244X 11073756 |
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