Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model
| العنوان: | Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model |
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| المؤلفون: | Alessandra De Simone, Raffaella Ravizza, Francesco Piccinini, Marzia B. Gariboldi, Elena Monti, Emanuela Marras, Gianpaolo Perletti |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | Spandidos Publications, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Antimetabolites, Antineoplastic, Cancer Research, Protein Kinase C-alpha, Cell Survival, Cell, HL-60 Cells, DNA Fragmentation, Drug resistance, Biology, Transfection, Proto-Oncogene Mas, medicine, Humans, Cytotoxic T cell, heterocyclic compounds, Protein Kinase C, Oncogene, Cytarabine, food and beverages, biochemical phenomena, metabolism, and nutrition, Cell cycle, medicine.disease, Isoenzymes, carbohydrates (lipids), Leukemia, medicine.anatomical_structure, Proto-Oncogene Proteins c-bcl-2, Oncology, Drug Resistance, Neoplasm, Cell culture, Apoptosis, Cancer research, lipids (amino acids, peptides, and proteins) |
| الوصف: | 1-beta-D-arabinofuranosylcytosine (ara-C) is an antimetabolite used for the treatment of acute myelogenous leukemia. The ability of ara-C to kill neoplastic cells has been correlated to the induction of apoptosis. The clinical use of ara-C is limited by the development of drug resistance. Alterations in drug-induced apoptosis play a critical role in ara-C resistance. In particular, the proto-oncogene bcl-2 has been implicated in this phenomenon. To better understand the molecular basis of the role of bcl-2 in ara-C resistance, we investigated the relationship between the cytotoxic effect of ara-C, the expression levels and the subcellular localization of bcl-2 in three human leukemic cell lines (HL-60, KG1, J111). We have also evaluated the effects of ara-C on the J111 leukemic cell line (showing the lowest levels of Bcl-2 and the highest sensitivity to ara-C) overexpressing the bcl-2 oncogene. The model we developed here will allow further studies on the role of post-translational events involving bcl-2 (such as translocation and/or phosphorylation) in the cellular response to ara-C treatment. |
| تدمد: | 1791-2423 1019-6439 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4c5cc50cfb3a42a6bb34accd6e540b1Test https://doi.org/10.3892/ijo.18.6.1245Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....c4c5cc50cfb3a42a6bb34accd6e540b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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