Grape seed extract attenuates arsenic-induced nephrotoxicity in rats
| العنوان: | Grape seed extract attenuates arsenic-induced nephrotoxicity in rats |
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| المؤلفون: | Xiaozhuan Liu, Xinjuan Pan, Zengli Yu, Ning Li, Xing Li, Xinjuan Yin, Yongchao Wang, Jiangong Zhang |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | Spandidos Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, food.ingredient, medicine.disease_cause, Nephrotoxicity, chemistry.chemical_compound, food, Immunology and Microbiology (miscellaneous), Internal medicine, medicine, oxidative stress, grape seed extract, TGF-β/Smad, Blood urea nitrogen, chemistry.chemical_classification, Reactive oxygen species, NADPH oxidase, biology, business.industry, nephrotoxicity, NOX4, Articles, General Medicine, chronic arsenic exposure, Endocrinology, chemistry, Grape seed extract, biology.protein, business, Oxidative stress, Nicotinamide adenine dinucleotide phosphate |
| الوصف: | Oxidative stress is a recognized factor in nephrotoxicity induced by chronic exposure to inorganic arsenic (As). Grape seed extract (GSE) possesses antioxidant properties. The present study was designed to evaluate the beneficial effects of GSE against arsenic-induced renal injury. Healthy, male Sprague-Dawley rats were exposed to As in drinking water (30 ppm) with or without GSE (100 mg/kg) for 12 months. The serum proinflammatory cytokine levels and mRNA expression levels of fibrogenic markers in the renal tissues were evaluated using enzyme-linked immunosorbent assay and quantitative polymerase chain reaction, respectively. The protein expression levels of nicotinamide adenine dinucleotide phosphate (NADPH) subunits, transforming growth factor-β1 (TGF-β1) and phosphorylated Smad2/3 (pSmad2/3) were assessed using western blot analysis. The results demonstrated that cotreatment with GSE significantly improved renal function, as demonstrated by the reductions in relative kidney weight (% of body weight) and blood urea nitrogen, and the increase in the creatinine clearance capacity. GSE attenuated the As-induced changes in the serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β and the mRNA levels of TGF-β1, α-smooth muscle actin (α-SMA), connective tissue growth factor (CTGF) and fibronectin (FN) in renal tissue. Furthermore, administration of GSE markedly reduced As-stimulated reactive oxygen species (ROS) production and Nox activity, as well as the protein expression levels of the NADPH subunits (Nox2, p47phox and Nox4). In addition, GSE cotreatment was correlated with a significant reduction in TGF-β/Smad signaling, as demonstrated by the decreased protein levels of TGF-β1 and pSmad2/3 in renal tissue. This study indicated that GSE may be a useful agent for the prevention of nephrotoxicity induced by chronic exposure to As. GSE may exert its effects through the suppression of Nox and inhibition of TGF-β/Smad signaling activation. |
| تدمد: | 1792-1015 1792-0981 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d5dd7a10ccc6267a93e796599998176Test https://doi.org/10.3892/etm.2013.1381Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8d5dd7a10ccc6267a93e796599998176 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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