Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone)
| العنوان: | Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone) |
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| المؤلفون: | Hiroki Wakabayashi, N Satoh, Hiroshi Kaneko, Yoshifumi Kawarada, N Tsuge, A Muraki, Kunio Nakashima, Hiroaki Ikeda, K Yonemaru, Hiroshige Hibasami |
| المصدر: | International Journal of Molecular Medicine. |
| بيانات النشر: | Spandidos Publications, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Mitoguazone, Time Factors, Spermine, Antineoplastic Agents, Apoptosis, Breast Neoplasms, DNA Fragmentation, Biology, chemistry.chemical_compound, Cyclin D1, Polyamines, Tumor Cells, Cultured, Genetics, Humans, RNA, Messenger, Dose-Response Relationship, Drug, Cell Cycle, General Medicine, Cell cycle, Flow Cytometry, Molecular biology, Gene Expression Regulation, Neoplastic, Spermidine, chemistry, Cancer cell, Growth inhibition, Polyamine, Cell Division |
| الوصف: | Polyamines are considered to be important intracellular molecules for the proliferation of the cancer cells. In this study, effects of methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), a potent inhibitor of the polyamine biosynthetic pathway, on the growth and cell cycle of T-47D human breast cancer cells were investigated. MGBCP dose-dependently inhibited the growth of T-47D cells, in which the contents of spermine, spermidine and putrescine decreased concomitantly. The gene expression of cyclin D1 was also repressed by the MGBCP treatment. The MGBCP-treated cells clearly exhibited morphological changes indicating the blebbing and chromatin condensation which are characteristic of apoptosis. Flow cytometric analysis showed hypo-diploid subpopulations due to apoptotic cells, and characteristic oligonucleosomal-sized DNA fragments were clearly observed for MGBCP-treated cells as the concentration of the drug was increased. These findings suggest that the inhibition of polyamine synthesis results in the repressions of cyclin D1 expression and cell cycle progression, eventually inducing apoptosis in these human breast cancer cells. |
| تدمد: | 1791-244X 1107-3756 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::611f40fd706a5e0672bdf9ae55d572fdTest https://doi.org/10.3892/ijmm.1.6.931Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....611f40fd706a5e0672bdf9ae55d572fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1791244X 11073756 |
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