Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas
| العنوان: | Mutational analysis of N-ras, p53, p16INK4a, p14ARF and CDK4 genes in primary human malignant mesotheliomas |
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| المؤلفون: | Klaus-Michael Müller, Qamar Rahman, Thilo Papp, Elke Dopp, Holger Schipper, Dieter G. Weiss, Dietmar Schiffmann, Heidi Pemsel, Ralf Bastrop, Thorsten Wiethege |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | Spandidos Publications, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Tumor suppressor gene, Neoplasms, Mesothelial, DNA Mutational Analysis, Loss of Heterozygosity, Biology, Polymerase Chain Reaction, Exon, Gene Frequency, p14arf, CDKN2A, Proto-Oncogene Proteins, Tumor Suppressor Protein p14ARF, Genotype, Tumor Cells, Cultured, medicine, Humans, Point Mutation, neoplasms, Gene, Polymorphism, Single-Stranded Conformational, Aged, Genes, p16, Point mutation, Cyclin-Dependent Kinase 4, Proteins, Cancer, Middle Aged, Genes, p53, medicine.disease, Cyclin-Dependent Kinases, Genes, ras, Oncology, Cancer research, Female |
| الوصف: | Nineteen specimens from primary human malignant mesotheliomas obtained from 19 patients were screened for activating point mutations in the oncogenes N-ras and CDK4 by combined RFLP-PCR/SSCP analysis. In addition, all tumours were screened for deletions and point mutations in the tumour suppressor genes p53, p16 INK4a (CDKN2A) and p14 ARF (exon-18) by combined multiplex-PCR/SSCP analysis. No mutations were found in N-ras, p53 and CDK4. Three tumours displayed homozygous deletion (co-deletion of exons 1, 2 and 3) of p16 INK4a . One of them displayed additional homozygous deletion of p14 ARF (exon-If)). Two silent point mutations and 2 polymorphisms were found in p16 INK4a in 3 tumours. Our preliminary data indicate that disarrangement of the Rb1 pathway may be involved in mesothelioma formation. |
| تدمد: | 1791-2423 1019-6439 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb2e54bacd7a7ddf3ac4f4adbbefde15Test https://doi.org/10.3892/ijo.18.2.425Test |
| رقم الانضمام: | edsair.doi.dedup.....cb2e54bacd7a7ddf3ac4f4adbbefde15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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