siRNA-mediated silencing of the 37/67-kDa high affinity laminin receptor in Hep3B cells induces apoptosis
| العنوان: | siRNA-mediated silencing of the 37/67-kDa high affinity laminin receptor in Hep3B cells induces apoptosis |
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| المؤلفون: | Duncan R. Smith, Tharinee Susantad |
| المصدر: | Cellular & Molecular Biology Letters |
| بيانات النشر: | SP Versita, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Cell Survival, Apoptosis, Biochemistry, Receptors, Laminin, chemistry.chemical_compound, RNA interference, Laminin, Annexin, Ribosomal protein, LAMR1, Cell Line, Tumor, Gene silencing, Animals, Humans, Viability assay, Propidium iodide, RNA, Small Interfering, Molecular Biology, Ribosomal, Laminin receptor, biology, Silencing, p40, Cell Biology, Molecular biology, Cell biology, chemistry, Liver, siRNA, biology.protein, Research Article |
| الوصف: | The laminin-binding protein, variously called the 37/67-kDa high affinity laminin receptor or p40, mediates the attachment of normal cells to the laminin network, and also has a role as a ribosomal protein. Over-expression of this protein has been strongly correlated with the metastatic phenotype. However, few studies have investigated the cellular consequence of the ablation of this gene’s expression. To address this issue, the expression of the 37/67-kDa high affinity laminin receptor was knocked out with several siRNA constructs via RNA interference in transformed liver (Hep3B) cells. In each case where the message was specifically ablated, apoptosis was induced, as determined by annexin V/propidium iodide staining, and by double staining with annexin V and an antibody directed against the 37/67-kDa high affinity laminin receptor. These results suggest that this protein plays a critical role in maintaining cell viability. |
| اللغة: | English |
| تدمد: | 1689-1392 1425-8153 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cb75d7f7e38d26f57ceb62153c4c3a5Test http://europepmc.org/articles/PMC6275979Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9cb75d7f7e38d26f57ceb62153c4c3a5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16891392 14258153 |
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