التفاصيل البيبلوغرافية
| العنوان: |
Effect of Follicle Size on In Vitro Production of Steroids and Insulin-Like Growth Factor (IGF)-I, IGF-II, and the IGF-Binding Proteins by Equine Ovarian Granulosa Cells1 |
| المؤلفون: |
Tonya R. Davidson, Connie S. Chamberlain, Tamara S. Bridges, Leon J. Spicer |
| المساهمون: |
Tonya R. Davidson, Connie S. Chamberlain, Tamara S. Bridges, Leon J. Spicer |
| المصدر: |
https://doi.org/10.1095/biolreprod66.6.1640Test. |
| بيانات النشر: |
Society for the Study of Reproduction |
| سنة النشر: |
2002 |
| المجموعة: |
BioOne Online Journals |
| جغرافية الموضوع: |
world |
| الوصف: |
Little is known regarding the hormonal regulation of granulosa cell steroidogenesis and the ovarian insulin-like growth factor (IGF) system in the mare. The objectives of this study were to determine, first, if estradiol, insulin, and/or FSH affect steroid production by equine granulosa cells (experiment 1) and, second, if the components of the IGF system are produced by equine granulosa cells in culture as well as whether estradiol, insulin, and/or FSH affects IGF and/or IGF-binding protein (IGFBP) production by equine granulosa cells (experiment 2). Granulosa cells from small (6–15 mm), medium (16–25 mm), and large (25–48 mm) follicles were collected from cyclic mares (n = 14), cultured for 2 days in medium containing 10% fetal calf serum, washed, and then treated for an additional 2 days in serum-free medium with or without added hormones. In experiment 1, large-follicle granulosa cells produced less progesterone and more estradiol than did medium- and/or small-follicle granulosa cells (P < 0.05). Progesterone production was inhibited (P < 0.05) by FSH and insulin in small- and medium- but not in large-follicle granulosa cells; estradiol was without effect. Insulin increased (P < 0.05) estradiol production in small- and medium-follicle granulosa cells but had no effect in large-follicle granulosa cells. In experiment 2, IGF-I production was inhibited (P < 0.05) by insulin across all follicle sizes but was not affected by estradiol or FSH. Granulosa cells of medium and large follicles produced more IGF-II than did granulosa cells of small follicles (P < 0.05). Insulin and FSH inhibited (P < 0.05) IGF-II production by granulosa cells of large and medium but not of small follicles; estradiol was without effect. Only IGFBP-2 and -5 were produced by equine granulosa cells. Production of IGFBP-2 was less (P < 0.10) in granulosa cells of large versus those of small and medium follicles, whereas medium-follicle granulosa cells produced more (P < 0.05) IGFBP-5 than did small- or large-follicle ... |
| نوع الوثيقة: |
text |
| وصف الملف: |
text/HTML |
| اللغة: |
English |
| DOI: |
10.1095/biolreprod66.6.1640 |
| الإتاحة: |
https://doi.org/10.1095/biolreprod66.6.1640Test |
| حقوق: |
All rights reserved. |
| رقم الانضمام: |
edsbas.273D6435 |
| قاعدة البيانات: |
BASE |
