دورية أكاديمية
Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile
| العنوان: | Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile |
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| المؤلفون: | Idrissi, Sarah Janati, Bourhis, Daniel Le, Lefevre, Antoine, Emond, Patrick, Le Berre, Laurene, Desnoës, Olivier, Joly, Thierry, Buff, Samuel, Freret, Sandrine, Schibler, Laurent, Salvetti, Pascal, Elis, Sébastien |
| المساهمون: | Allice, Université de Tours, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), ISARA-Lyon, Interactions Cellules Environnement - UR (ICE), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Physiologie de la reproduction et des comportements Nouzilly (PRC), Institut Français du Cheval et de l'Equitation Saumur -Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | ISSN: 0006-3363. |
| بيانات النشر: | HAL CCSD Society for the Study of Reproduction |
| سنة النشر: | 2021 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | bovine, in vitro embryo production, N-3 PUFA, cryopreservation, embryo quality, lipidomic, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDE]Environmental Sciences, [SDV]Life Sciences [q-bio], [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism |
| الوصف: | International audience ; Abstract Embryo lipid profile is affected by in vitro culture conditions, that lead to an increase in lipids. Efforts have been made to optimize embryo lipid composition as it is associated with their quality. The objective of this study was to evaluate whether the diet supplementation of donor cows (n-3 or n-6 PUFA), or the slow freezing protocols (ethylene glycol sucrose EG-S vs. glycerol trehalose GLY-TRE), or the physiological stage of the donor (nulliparous heifers vs. primiparous lactating cows) may impact the bovine embryo lipid profile. Lipid extracts of 97 embryos were individually analysed by liquid chromatography-high resolution mass spectrometry, highlighting 246 lipids including 85% being overabundant in cow embryos compared to heifer embryos. Among 105 differential lipids, 72 were overabundant after EG-S protocol, including a single glycerophosphate PA(32:1) representing 27.3% of the significantly modulated lipids, suggesting that it is degraded when GLY-TRE is used. No lipids were different according to the n-3 or n-6 supplementation of the donor cows. In conclusion, the embryonic lipid profile was mainly affected by the physiological stage of the donors and the slow freezing protocols. The overabundance of lipids in lactating cow embryos and the resulting lower quality of these embryos is consistent with the lower pregnancy rate observed in cows compared to heifers. Unlike GLY-TRE protocol, EG-S freezing allowed to preserve glycerophospholipids potentially improving the slow freezing of in vitro-produced embryos. Further studies are required to modulate embryo quality and freezability by modulating the lipidome and integrating all stages of embryonic production. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03413303; https://hal.inrae.fr/hal-03413303Test |
| DOI: | 10.1093/biolre/ioab198 |
| الإتاحة: | https://doi.org/10.1093/biolre/ioab198Test https://hal.inrae.fr/hal-03413303Test |
| رقم الانضمام: | edsbas.F5EE8C7D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/biolre/ioab198 |
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