Hes1andHes5Activities Are Required for the Normal Development of the Hair Cells in the Mammalian Inner Ear
| العنوان: | Hes1andHes5Activities Are Required for the Normal Development of the Hair Cells in the Mammalian Inner Ear |
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| المؤلفون: | Alexandre Aubert, Ryoichiro Kageyama, Jiping Qiu, Azel Zine, François Guillemot, François de Ribaupierre, Stavros Therianos |
| المصدر: | Journal of Neuroscience, vol. 21, no. 13, pp. 4712-4720 |
| بيانات النشر: | Society for Neuroscience, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Heterozygote, Labyrinth Supporting Cells, Cell Count, Nerve Tissue Proteins, Biology, Mice, Animals, Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation/genetics, Cochlea/cytology, Cochlea/embryology, Crosses, Genetic, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Ear, Inner/cytology, Ear, Inner/embryology, Hair Cells, Auditory/metabolism, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, Homozygote, Immunohistochemistry, In Situ Hybridization, Mice, Mutant Strains, Nerve Tissue Proteins/metabolism, RNA, Messenger/metabolism, Repressor Proteins/genetics, Repressor Proteins/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/genetics, Transcription Factors/metabolism, Up-Regulation, Hair Cells, Auditory, otorhinolaryngologic diseases, medicine, Inner ear, RNA, Messenger, ARTICLE, HES1, Cochlea, Homeodomain Proteins, Hair cell differentiation, General Neuroscience, Cell Differentiation, Anatomy, Kinocilium, Cell biology, DNA-Binding Proteins, Repressor Proteins, medicine.anatomical_structure, Ear, Inner, Transcription Factor HES-1, sense organs, Saccule, Hair cell, Transcription Factors |
| الوصف: | The mammalian inner ear contains two sensory organs, the cochlea and vestibule. Their sensory neuroepithelia are characterized by a mosaic of hair cells and supporting cells. Cochlear hair cells differentiate in four rows: a single row of inner hair cells (IHCs) and three rows of outer hair cells (OHCs). Recent studies have shown that Math1, a mammalian homolog ofDrosophila atonalis a positive regulator of hair cell differentiation. The basic helix–loop–helix (bHLH) genesHes1andHes5(mammalianhairyandEnhancer-of-splithomologs) can influence cell fate determination by acting as negative regulators to inhibit the action of bHLH-positive regulators. We show by using reverse transcription-PCR analysis thatHes1,Hes5, andMath1are expressed in the developing mouse cochleae.In situhybridization revealed a widespread expression ofHes1in the greater epithelial ridge (GER) and in lesser epithelial ridge (LER) regions.Hes5is predominantly expressed in the LER, in supporting cells, and in a narrow band of cells within the GER.Examination of cochleae fromHes1−/−mice showed a significant increase in the number of IHCs, whereas cochleae fromHes5−/−mice showed a significant increase in the number of OHCs. In the vestibular system, targeted deletion ofHes1and to a lesser extentHes5lead to formation of supernumerary hair cells in the saccule and utricle.The supernumerary hair cells in the mutant mice showed an upregulation of Math1. These data indicate thatHes1andHes5participate together for the control of inner ear hair cell production, likely through the negative regulation of Math1. |
| وصف الملف: | application/pdf |
| تدمد: | 1529-2401 0270-6474 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10f03adbbf39c5ac0a21d26613d5a16cTest https://doi.org/10.1523/jneurosci.21-13-04712.2001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....10f03adbbf39c5ac0a21d26613d5a16c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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