Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
العنوان: | Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum |
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المؤلفون: | Fábio de Barros, Fábio Pinheiro, Clarisse Palma-Silva, Salvatore Cozzolino |
المساهمون: | Pinheiro, F., Palma Silva, C., de Barros, F., Cozzolino, Salvatore |
المصدر: | Genetics and Molecular Biology v.32 n.2 2009 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 32, Issue: 2, Pages: 337-339, Published: 27 MAR 2009 Scopus-Elsevier Genetics and Molecular Biology, Vol 32, Iss 2, Pp 337-339 (2009) |
بيانات النشر: | Sociedade Brasileira de Genética, 2009. |
سنة النشر: | 2009 |
مصطلحات موضوعية: | Genetics, Orchidaceae, lcsh:QH426-470, biology, Short Communication, short tandem repeat, Epidendrum, Locus (genetics), biology.organism_classification, Plant Genetics, Loss of heterozygosity, lcsh:Genetics, Genus, Evolutionary biology, Microsatellite, Allele, Cross amplification, cross-amplification, Molecular Biology |
الوصف: | In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus. |
وصف الملف: | text/html; STAMPA |
اللغة: | English |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a1fadfc7fb14d2a1dc849b3ace4f63dTest http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....0a1fadfc7fb14d2a1dc849b3ace4f63d |
قاعدة البيانات: | OpenAIRE |
الوصف غير متاح. |