Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

التفاصيل البيبلوغرافية
العنوان: Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
المؤلفون: Fábio de Barros, Fábio Pinheiro, Clarisse Palma-Silva, Salvatore Cozzolino
المساهمون: Pinheiro, F., Palma Silva, C., de Barros, F., Cozzolino, Salvatore
المصدر: Genetics and Molecular Biology v.32 n.2 2009
Genetics and Molecular Biology
Sociedade Brasileira de Genética (SBG)
instacron:SBG
Genetics and Molecular Biology, Volume: 32, Issue: 2, Pages: 337-339, Published: 27 MAR 2009
Scopus-Elsevier
Genetics and Molecular Biology, Vol 32, Iss 2, Pp 337-339 (2009)
بيانات النشر: Sociedade Brasileira de Genética, 2009.
سنة النشر: 2009
مصطلحات موضوعية: Genetics, Orchidaceae, lcsh:QH426-470, biology, Short Communication, short tandem repeat, Epidendrum, Locus (genetics), biology.organism_classification, Plant Genetics, Loss of heterozygosity, lcsh:Genetics, Genus, Evolutionary biology, Microsatellite, Allele, Cross amplification, cross-amplification, Molecular Biology
الوصف: In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
وصف الملف: text/html; STAMPA
اللغة: English
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a1fadfc7fb14d2a1dc849b3ace4f63dTest
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....0a1fadfc7fb14d2a1dc849b3ace4f63d
قاعدة البيانات: OpenAIRE