Mechanism of curcumin inhibiting pyroptosis in infectious acute lung injury through NLRP3 inflammatory pathway
| العنوان: | Mechanism of curcumin inhibiting pyroptosis in infectious acute lung injury through NLRP3 inflammatory pathway |
|---|---|
| المؤلفون: | Qiwen WU, Bo WU, Liandi LIU, Xiaoli LAI, Yongjia DENG, Xi HUANG, Liankun ZENG, Yan BAI, Yeping YAO |
| المصدر: | Food Science and Technology v.42 2022 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA Food Science and Technology, Volume: 42, Article number: e45122, Published: 08 AUG 2022 |
| بيانات النشر: | Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | acute lung injury, curcumin, nLRP3, Food Science, Biotechnology, cell pyroptosis |
| الوصف: | Purpose of this study the mechanism of curcumin inhibiting pyroptosis in infectious acute lung injury through NLRP3 inflammatory pathway. In this study, SPF SD male rats were randomly divided into blank control group, curcumin group, lipopolysaccharide (LPS) group, LPS + curcumin group, LPS + Nigerin treatment group, LPS + Nigerin + curcumin treatment group. Three in each group. Animal models were established according to the experimental design. Blood samples were collected after anesthesia, and the lung wet/dry weight ratio was calculated. TUNEL was used to detect pyroptosis of alveolar macrophages in left lower lobe, and Elisa was used to detect the expression levels of inflammatory factors IL-1β and IL-18 in left lung tissue. Western Blot was used to detect the expression of pyroptosis-related proteins such as NLRP3, Caspase1 and GSDMD in right lung alveolar lavage fluid. Compared with the blank control group, the lung wet weight and lung dry weight of the curcumin group were significantly increased (P < 0.01, P < 0.05). Compared with the LPS group, the lung wet weight and lung dry weight of the curcumin group There was a statistical difference in weight increase (P < 0.01). Compared with the blank control group, the apoptosis rate of lung tissue in the curcumin group, LPS group and LPS+ nigericin group was significantly increased (P < 0.01). Compared with the LPS + nigericin group, the lung tissue apoptosis rate in the LPS + curcumin + nigericin group was decreased, but there was no statistical difference (P > 0.05). LPS and LPS combined with Nigerian bacteriocin can cause pyroptosis in lung tissue. Curcumin can inhibit pyroptosis of lung tissue macrophages caused by LPS and LPS combined with Nigerian bacteriocin, and reduce inflammatory response. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00546c59412471abdfd8eab68ca589d7Test http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101269Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....00546c59412471abdfd8eab68ca589d7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |