Qilian Huaji decoction exerts an anti-cancer effect on hepatocellular carcinoma by upregulating miR-122
| العنوان: | Qilian Huaji decoction exerts an anti-cancer effect on hepatocellular carcinoma by upregulating miR-122 |
|---|---|
| المؤلفون: | Dan Zhao, Qingyong Ma, Wen An, Xuejun Jiang, Yongsheng Zhou, Xiaoping Gao |
| المصدر: | Food Science and Technology, Volume: 42, Article number: e61620, Published: 09 JUL 2021 Food Science and Technology v.42 2022 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA Food Science and Technology, Issue: ahead, Published: 09 JUL 2021 Food Science and Technology (2021) |
| بيانات النشر: | Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cell cycle checkpoint, medicine.diagnostic_test, Nutrition. Foods and food supply, Cell growth, Chemistry, Hepatocellular carcinoma, Qilian Huaji Decoction, Cell cycle, digestive system diseases, Flow cytometry, miR-122, Western blot, Apoptosis, In vivo, MiR-122, Cancer research, medicine, T1-995, TX341-641, Technology (General), anti-cancer, Food Science, Biotechnology |
| الوصف: | Qilian Huaji Decoction (QLHJD) is a mixture of 13 herbs. The aim of this study is to observe the efficacy of QLHJD against hepatocellular carcinoma (HCC). The H22 transplanted mouse models were established to explore the effect of QLHJD on HCC in vivo. QLHJD-containing serum (QCS) was prepared using traditional Chinese medicine serum pharmacology methods. After QCS treatment, cell proliferation, cell cycle and apoptosis in HepG2 cells were assessed by MTT and flow cytometry, respectively. miRNA expression profiles were determined using qPCR arrays. miR-122 was examined by qPCR in HCC tissue samples and cells. AKT3 protein expression was detected by Western blot. We found that QLHJD significantly inhibited the growth of H22 tumor and improve immune function in vivo. In vitro, QLHJD inhibited proliferation by inducing cell cycle arrest at the S phase, and induced apoptosis in HepG2 cells. The miRNA expression profiles were altered and the reduced miR-122 levels in HepG2 cell lines were up-regulated compared with control group, accompanied by a decrease in WNT1 expression. In conclusion, QLHJD could inhibit the growth of HCC by restoring the aberrant expression of miR-122, which may be one of the mechanisms whereby QLHJD exerts its effect on HCC. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c427dd2f807ec96cd023e59979c146abTest http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100634&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c427dd2f807ec96cd023e59979c146ab |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |