رسالة جامعية
Development of Methodology for Rapid Bacterial Detection in Complex Matrices Using SERS
| العنوان: | Development of Methodology for Rapid Bacterial Detection in Complex Matrices Using SERS |
|---|---|
| المؤلفون: | Tucker, Madeline |
| المصدر: | Masters Theses. |
| بيانات النشر: | ScholarWorks@UMass Amherst. |
| المجموعة: | University of Massachusetts, Amherst |
| مصطلحات موضوعية: | Bacteria, Surface Enhanced Raman, Complex Matrices, Food Chemistry, Food Microbiology |
| الوصف: | Fresh foods, including meats and produce are the fastest growing market in the supermarket and the class of foods most likely to cause a bacterial foodborne illness. As the rate of consumption of perishable products increases, rapid detection of pathogens within the food supply becomes a critical issue. Current methods used for the detection of bacteria that cause food-borne illnesses are time consuming, expensive and often require selective enrichment. In this study we adapted a separation technique originally developed for PCR to extract bacteria from ground beef using β-cyclodextrin (β-CD) and milk protein coated activated carbon (MP-CAC) as filtration agents. The recovered bacteria were bound to a gold slide via a 3-mercaptophenylboronic acid (3-MPBA) sandwich assay and detected with Surface Enhanced Raman Spectroscopy (SERS). The 3-MPBA sandwich assay used with the separation technique allowed detection of Salmonella enterica Enteritidis (BAA-1045), separated from a ground beef matrix, as low as 1x102 CFU/g. Detection at this level was accomplished in less than 8 hours, significantly faster than plate count or enrichment methods that require multiple days. Previously, SERS has been used to detect bacteria within simple matrices; this is the first study to have utilized SERS bacterial detection in a ground beef. |
| Original Identifier: | oai:scholarworks.umass.edu:masters_theses_2-1656 |
| نوع الوثيقة: | Text |
| وصف الملف: | application/pdf |
| الإتاحة: | https://scholarworks.umass.edu/masters_theses_2/670Test https://scholarworks.umass.edu/context/masters_theses_2/article/1656/viewcontent/Madeline_Tucker_Thesis_Final_revision_3.pdfTest |
| رقم الانضمام: | edsndl.UMASS.oai.scholarworks.umass.edu.masters.theses.2.1656 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |