Bone Marrow Mesenchymal Stem Cells and Electroacupuncture Downregulate the Inhibitor Molecules and Promote the Axonal Regeneration in the Transected Spinal Cord of Rats
| العنوان: | Bone Marrow Mesenchymal Stem Cells and Electroacupuncture Downregulate the Inhibitor Molecules and Promote the Axonal Regeneration in the Transected Spinal Cord of Rats |
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| المؤلفون: | Qing Yan, Ying Ding, Yuan Shan Zeng, Si Fan Huang, Yu Jiao Zhang, Shirlene Wang, Jing Wen Ruan, Wen Jie Li, Hongxin Dong, Xiang Zeng, Yanqing Zhang |
| المصدر: | Cell Transplantation, Vol 20 (2011) |
| بيانات النشر: | SAGE Publishing, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Electroacupuncture, medicine.medical_treatment, Biomedical Engineering, Down-Regulation, lcsh:Medicine, Bone Marrow Cells, Mesenchymal Stem Cell Transplantation, Rats, Sprague-Dawley, Glial Fibrillary Acidic Protein, medicine, Animals, Spinal Cord Injuries, Transplantation, Glial fibrillary acidic protein, biology, business.industry, Regeneration (biology), Mesenchymal stem cell, lcsh:R, Mesenchymal Stem Cells, Cell Biology, Anatomy, Spinal cord, Immunohistochemistry, Survival Analysis, Retrograde tracing, Axons, Nerve Regeneration, Rats, Disease Models, Animal, medicine.anatomical_structure, Chondroitin Sulfate Proteoglycans, Corticospinal tract, biology.protein, Female, business |
| الوصف: | Our previous study has reported that electroacupuncture (EA) promotes survival, differentiation of bone marrow mesenchymal stem cells (MSCs), and functional improvement in spinal cord-transected rats. In this study, we further investigated the structural bases of this functional improvement and the potential mechanisms of axonal regeneration in injured spinal cord after MSCs and EA treatment. Five experimental groups, 1) sham control (Sham-control); 2) operated control (Op-control); 3) electroacupuncture treatment (EA); 4) MSCs transplantation (MSCs), and 5) MSCs transplantation combined with electroacupuncture (MSCs + EA), were designed for this study. Western blots and immunohistochemical staining were used to assess the fibrillary acidic protein (GFAP) and chondroitin sulfate proteoglycans (CSPGs) proteins expression. Basso, Beattie, Bresnahan (BBB) locomotion test, cortical motor evoked potentials (MEPs), and anterograde and retrograde tracing were utilized to assess cortical-spinal neuronal projection regeneration and functional recovery. In the MSCs + EA group, increased labeling descending corticospinal tract (CST) projections into the lesion site showed significantly improved BBB scales and enhanced motor evoked potentials after 10 weeks of MSCs transplant and EA treatment. The structural and functional recovery after MSCs + EA treatment may be due to downregulated GFAP and CSPGs protein expression, which prevented axonal degeneration as well as improved axonal regeneration. |
| اللغة: | English |
| تدمد: | 1555-3892 0963-6897 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0125d9e437ab8de3fd7bf9398ae62df8Test https://doaj.org/article/18cd157f179d471bb8a1c49bc63bc718Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0125d9e437ab8de3fd7bf9398ae62df8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15553892 09636897 |
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