التفاصيل البيبلوغرافية
| العنوان: |
Effects of Tissue Factor Pathway Inhibitor-2 Expression on Biological Behavior of BeWo and JEG-3 Cell Lines. |
| المؤلفون: |
Zhou, Qiongjie, Xiong, Yu, Chen, Yan, Du, Yingying, Zhang, Jin, Mu, Jingui, Guo, Qisang, Wang, Huijun, Ma, Duan, Li, Xiaotian |
| المصدر: |
Clinical & Applied Thrombosis/Hemostasis; Sep2012, Vol. 18 Issue 5, p526-533, 8p |
| مستخلص: |
Objectives: To investigate the effect of tissue factor pathway inhibitor-2 (TFPI-2) expression on biological behavior of BeWo and JEG-3 cell lines. Material and Methods: The expression of TFPI-2 in BeWo and JEG-3 cells was upregulated by pEGFP-N3-TFPI-2 and downregulated by small interference RNA transfection, confirmed by Western blotting assay and real-time polymerase chain reaction (RT-PCR). Boyden chamber, Cell Counting Kit-8 (CCK-8), and Hoechst 33258/terminal deoxynucleotidyltransferase-mediated UTP end labeling (TUNEL) assays were used for migration, invasion, and proliferation/apoptosis analysis, respectively. Results: In Western blotting and RT-PCR assay, protein and messenger RNA (mRNA) expression of TFPI-2 in transfected BeWo and JEG-3 cells were confirmed. Expression of TFPI-2 inhibited BeWo and downregulated JEG-3 cell migration, invasion, proliferation, and induced apoptosis (P < .05) in Boyden chamber, CCK-8, Hoechst 33258, and TUNEL detection, respectively. Conclusions: TFPI-2 expression caused invasion and proliferation impair and induced apoptosis in TFPI-2 regulated BeWo and JEG-3 cells. It provides a clue for potential role of TFPI-2 in trophoblast. [ABSTRACT FROM PUBLISHER] |
|
Copyright of Clinical & Applied Thrombosis/Hemostasis is the property of Sage Publications Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
