Human Pancreatic Islet Preparations Release HMGB1: (Ir)Relevance for Graft Engraftment
| العنوان: | Human Pancreatic Islet Preparations Release HMGB1: (Ir)Relevance for Graft Engraftment |
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| المؤلفون: | Marina Scavini, Zhidong Ling, Lorenzo Piemonti, Valeria Sordi, Olle Korsgren, Barbara Celona, Leda Racanicchi, Raffaella Melzi, Rita Nano, Antonio Secchi, Paola Maffi, Alessia Mercalli |
| المساهمون: | Nano, R, Racanicchi, L, Melzi, R, Mercalli, A, Maffi, P, Sordi, V, Ling, Z, Scavini, M, Korsgren, O, Celona, B, Secchi, Antonio, Piemonti, Lorenzo |
| المصدر: | Cell Transplantation, Vol 22 (2013) |
| بيانات النشر: | SAGE Publications, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Adult, Male, Interleukin-1beta, Islets of Langerhans Transplantation, Biomedical Engineering, lcsh:Medicine, chemical and pharmacologic phenomena, HMGB1, Bioinformatics, Fibrin Fibrinogen Degradation Products, Islets of Langerhans, Humans, Transplantation, Homologous, HMGB1 Protein, Cells, Cultured, Transplantation, geography, geography.geographical_feature_category, biology, lcsh:R, Toll-Like Receptors, Cell Biology, Middle Aged, Islet, Diabetes Mellitus, Type 2, Immunology, biology.protein, Cytokines, Female, Chemokines |
| الوصف: | High levels of donor-derived high-mobility group box 1 (HMGB1) protein have been associated with poor islet graft outcome in mouse models. The aim of our work was to determine whether HMGB1 released by human islets had independent proinflammatory effects that influence engraftment in humans. Human islet preparations contained and released HMGB1 in different amounts, as determined by Western blot and ELISA (median 17 pg/ml/IEQ/24 h; min–max 0–211, n = 74). HMGB1 release directly correlated with brain death, donor hyperamilasemia, and factors related to the pancreas digestion procedure (collagenase and digestion time). HMGB1 release was significantly positively associated with the release of other cytokines/chemokines, particularly with the highly released “proinflammatory” CXCL8/IL-8, CXCL1/GRO-α, and the IFN-γ-inducible chemokines CXCL10/IP-10 and CXCL9/MIG. HMGB1 release was not modulated by Toll-like receptor 2, 3, 4, 5, and 9 agonists or by exposure to IL-1β. When evaluated after islet transplantation, pretransplant HMGB1 release was weakly associated with the activation of the coagulation cascade (evaluated as serum cross-linked fibrin products), but not with the immediate posttransplant inflammatory response. Concordantly, HMGB1 did not affect short-term human islet function. Our data show that human islet HMGB1 release is a sign of “damaged” islets, although without any independent direct role in graft failure. |
| تدمد: | 1555-3892 0963-6897 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bac010a5c95e68d2c6819a1a08b975dfTest https://doi.org/10.3727/096368912x657783Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bac010a5c95e68d2c6819a1a08b975df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15553892 09636897 |
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