Posttraumatic Osteoarthritis Damage in Mice: From Histological and Micro–Computed Tomodensitometric Changes to Gait Disturbance
| العنوان: | Posttraumatic Osteoarthritis Damage in Mice: From Histological and Micro–Computed Tomodensitometric Changes to Gait Disturbance |
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| المؤلفون: | Alban Fouasson-Chailloux, Benoit Bodic, Julie Lesoeur, Joëlle Veziers, François Rannou, Yves Maugars, Claire Vinatier, Jérôme Guicheux, M. Masson, Marc Dauty, Benoit Metayer |
| المصدر: | Cartilage |
| بيانات النشر: | SAGE Publications, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Biomedical Engineering, Male mice, Physical Therapy, Sports Therapy and Rehabilitation, Walking, Osteoarthritis, Menisci, Tibial, Mice, Joint disease, Disease severity, Animals, Immunology and Allergy, Medicine, Gait, Clinical Research papers, business.industry, Gait Disturbance, medicine.disease, Mice, Inbred C57BL, medicine.anatomical_structure, Gait analysis, Anesthesia, business, Medial meniscus |
| الوصف: | Objectives Osteoarthritis is a painful joint disease responsible for walking impairment. Its quantitative assessment by gait analysis in mice may be a relevant and noninvasive strategy to assess the disease severity. In this study, we aimed to determine the severity of osteoarthritis at the tissular and gait levels in unilateral and bilateral posttraumatic murine osteoarthritis. Methods Twenty-four C57BL/6 male mice were randomly assigned to 3 groups ( n = 8/group): controls, unilateral surgery, and bilateral surgery. Posttraumatic osteoarthritis was induced unilaterally or bilaterally by destabilization of the medial meniscus. Gait analysis was performed weekly with the CatWalkTM XT system until the 16th week after surgery. After animal sacrifices, histological and micro–computed tomographic assessment was performed. Results Operated knees showed a significant increase in the histological score compared with controls ( P < 0.001). Calcified anterior medial meniscal bone volume was higher on the ipsilateral side after unilateral destabilization of the medial meniscus ( P < 0.001) and on both sides after bilateral intervention ( P < 0.01). One week after surgery, the mice mean speed decreased significantly in both operated groups ( P < 0.001 and P < 0.05). In the unilateral group, a significant increase in the contralateral hind print area appeared from week 4 to week 16. Conclusions While bilateral destabilization of the medial meniscus induced no detectable gait modification except 1 week after surgery, unilateral model was responsible for a gait disturbance on the contralateral side. Further studies are needed to better define the place of the CatWalkTM in the evaluation of mouse models of osteoarthritis. |
| تدمد: | 1947-6043 1947-6035 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67f66e88980123d3b9a785acfa46f224Test https://doi.org/10.1177/19476035211053821Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67f66e88980123d3b9a785acfa46f224 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19476043 19476035 |
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