Human neutrophilic and eosinophilic granulocytes display different levels of c-fos proto-oncogene expression: an in situ hybridization study
| العنوان: | Human neutrophilic and eosinophilic granulocytes display different levels of c-fos proto-oncogene expression: an in situ hybridization study |
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| المؤلفون: | H J Radzun, C Mäder, M R Parwaresch, Klaus Heidorn, H Kreipe |
| المصدر: | Journal of Histochemistry & Cytochemistry. 35:837-842 |
| بيانات النشر: | SAGE Publications, 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Histology, Myeloid, Neutrophils, Nucleic Acid Hybridization, In situ hybridization, Eosinophil, Biology, Granulocyte, Proto-Oncogene Mas, Molecular biology, Monocytes, Eosinophils, Blood cell, Haematopoiesis, medicine.anatomical_structure, Gene Expression Regulation, Proto-Oncogene Proteins, Proto-Oncogenes, medicine, Humans, RNA, Messenger, Myelopoiesis, Anatomy, Promyelocyte |
| الوصف: | The cellular homologue of the retroviral oncogene v-fos has been shown to be involved in cell differentiation of hematopoietic cells. By use of the human promyelocyte cell line HL-60, several in vitro differentiation studies suggested a selective activation of c-fos during monocytic differentiation of myeloid precursor cells. In contrast to these observations, we found high levels of c-fos mRNA in purified normal human granulocytes, whereas c-fos was only faintly expressed in blood monocytes. In situ hybridization revealed that the high level of c-fos expression is restricted to neutrophilic granulocytes, whereas c-fos transcription is not detectable in eosinophilic granulocytes. These results indicate that in vitro differentiation systems can be misleading and may not reflect the in vivo situation. The high level of c-fos expression in neutrophilic granulocytes may be caused by superinduction due to the reduced capacity for protein synthesis in these cells. |
| تدمد: | 1551-5044 0022-1554 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::508d7962341d258dbe48a9f568675bf8Test https://doi.org/10.1177/35.8.3298425Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....508d7962341d258dbe48a9f568675bf8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15515044 00221554 |
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